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Literature summary for 1.97.1.1 extracted from

  • Kengen, S.W.M.; Rikken, G.B.; Hagen, W.R.; Van Ginkel, C.G.; Stams, A.J.M.
    Purification and characterization of (per)chlorate reductase from the chlorate-respiring strain GR-1 (1999), J. Bacteriol., 181, 6706-6711.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
degradation bacterial reduction of chlorate and perchlorate in water Azospira oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
Chlorate
-
Azospira oryzae
0.027
-
perchlorate
-
Azospira oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Azospira oryzae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe contains iron: 30.6 mol, based of apparent molecular mass of 420000 Da Azospira oryzae
Mo contains molybdenum: 2.9 mol, based of apparent molecular mass of 420000 Da Azospira oryzae
Se contains selenium 2.9 mol, based of apparent molecular mass of 420000 Da Azospira oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
alpha3,beta3, 3 * 95000 + 3* 40000, each dimer: alpha and beta contains 1 Mo, 1 Se and 10 Fe, SDS-PAGE Azospira oryzae
420000
-
SDS-PAGE Azospira oryzae

Organism

Organism UniProt Comment Textmining
Azospira oryzae
-
DSM 11199
-
Azospira oryzae GR-1
-
DSM 11199
-

Oxidation Stability

Oxidation Stability Organism
oxygen sensitive, aerobically stored extracts show a half-life of inactivation of 2 to 3 d Azospira oryzae

Purification (Commentary)

Purification (Comment) Organism
-
Azospira oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26.4
-
-
Azospira oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bromate + reduced electron acceptor
-
Azospira oryzae bromite + H2O + oxidized electron acceptor
-
?
bromate + reduced electron acceptor
-
Azospira oryzae GR-1 bromite + H2O + oxidized electron acceptor
-
?
chlorate + reduced electron acceptor methyl viologen Azospira oryzae chlorite + H2O + oxidized electron acceptor
-
?
chlorate + reduced electron acceptor methyl viologen Azospira oryzae GR-1 chlorite + H2O + oxidized electron acceptor
-
?
iodate + reduced electron acceptor
-
Azospira oryzae iodite + H2O + oxidized electron acceptor
-
?
iodate + reduced electron acceptor
-
Azospira oryzae GR-1 iodite + H2O + oxidized electron acceptor
-
?
nitrate + reduced electron acceptor
-
Azospira oryzae nitrite + H2O + oxidized electron acceptor
-
?
nitrate + reduced electron acceptor
-
Azospira oryzae GR-1 nitrite + H2O + oxidized electron acceptor
-
?
perchlorate + reduced electron acceptor
-
Azospira oryzae chlorate + H2O + oxidized electron acceptor
-
?
perchlorate + reduced electron acceptor
-
Azospira oryzae GR-1 chlorate + H2O + oxidized electron acceptor
-
?

Subunits

Subunits Comment Organism
hexamer alpha3,beta3, 3 * 95000 + 3* 40000, each dimer: alpha and beta contains 1 Mo, 1 Se and 10 Fe, SDS-PAGE Azospira oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Azospira oryzae