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Literature summary for 1.9.6.1 extracted from

  • Gates, A.J.; Richardson, D.J.; Butt, J.N.
    Voltammetric characterization of the aerobic energy-dissipating nitrate reductase of Paracoccus pantotrophus: exploring the activity of a redox-balancing enzyme as a function of electrochemical potential (2008), Biochem. J., 409, 159-168.
    View publication on PubMed
Search for more literature for 1.9.6.1

Inhibitors

Inhibitors Comment Organism Structure
azide mixed type inhibition Paracoccus pantotrophus
thiocyanate competitive Paracoccus pantotrophus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 nitrate pH 7, NapAB catalysed nitrate reduction driven by direct electron transfer from the electrode to NapAB, protein film voltammetry Paracoccus pantotrophus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 x * 16000 + x * 90000, SDS-PAGE Paracoccus pantotrophus
90000
-
 x * 16000 + x * 90000, SDS-PAGE Paracoccus pantotrophus

Organism

Organism UniProt Comment Textmining
Paracoccus pantotrophus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paracoccus pantotrophus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NapAB catalysed nitrate reduction driven by direct electron transfer from the electrode to NapAB, protein film voltammetry. Exploration of the nitrate reductase activity of purified NapAB as a function of electrochemical potential, substrate concentration and pH using protein film voltammetry. Nitrate reduction by NapAB occurs at potentials below approx. 0.1 V at pH 7. These are lower potentials than required for NarGH nitrate reduction. The potentials required for Nap nitrate reduction are also likely to require ubiquinol/ubiquinone ratios higher than are needed to activate the H+-pumping oxidases expressed during aerobic growth where Nap levels are maximal. Thus the operational potentials of Paracoccus pantotrophus NapAB are consistent with a productive role in redox balancing Paracoccus pantotrophus ?
-
 ?
nitrate + reduced methyl viologen
-
 Paracoccus pantotrophus nitrite + oxidized methyl viologen + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 16000 + x * 90000, SDS-PAGE Paracoccus pantotrophus

Synonyms

Synonyms Comment Organism
NapAB
-
 Paracoccus pantotrophus