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Literature summary for 1.8.99.B1 extracted from
- Sulfolobus solfataricus thiol redox puzzle: characterization of an atypical protein disulfide oxidoreductase (2013), Extremophiles, 18, 219-228.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Saccharolobus solfataricus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the X-ray crystallographic structure is solved at 1.80 A resolution | Saccharolobus solfataricus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19930 | - |
1 * 19930, calculated from sequence | Saccharolobus solfataricus |
| 20995 | - |
1 * 20995, light scattering and electrospray mass spectroscopy analyses | Saccharolobus solfataricus |
| 21000 | - |
light scattering and electrospray mass spectroscopy analyses | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein-dithiol + oxidized acceptor | Saccharolobus solfataricus | the enzyme is involved in disulfide bond formation | protein-disulfide + reduced acceptor | - |
? |  |
| protein-dithiol + oxidized acceptor | Saccharolobus solfataricus P2 | the enzyme is involved in disulfide bond formation | protein-disulfide + reduced acceptor | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| guanidine-HCl | [GuHCl]/2 value is 1.5 M | Saccharolobus solfataricus |
| urea | resistant to urea presenting a [urea]/2 value of 5 M | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q97Z21 | - |
- |
| Saccharolobus solfataricus P2 | Q97Z21 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein-dithiol + oxidized acceptor | the enzyme is involved in disulfide bond formation | Saccharolobus solfataricus | protein-disulfide + reduced acceptor | - |
? | |
| protein-dithiol + oxidized acceptor | the enzyme is involved in disulfide bond formation | Saccharolobus solfataricus P2 | protein-disulfide + reduced acceptor | - |
? | |
| [insulin]-disulfide + reduced dithiothreitol | the enzyme does not present isomerase activity | Saccharolobus solfataricus | [insulin]-dithiol + oxidized dithiothreitol | - |
? | |
| [insulin]-disulfide + reduced dithiothreitol | the enzyme does not present isomerase activity | Saccharolobus solfataricus P2 | [insulin]-dithiol + oxidized dithiothreitol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 19930, calculated from sequence | Saccharolobus solfataricus |
| monomer | 1 * 20995, light scattering and electrospray mass spectroscopy analyses | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Sso1120 | - |
Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
Tm-value | Saccharolobus solfataricus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharolobus solfataricus | calculated from sequence | - |
5.3 |
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Release 2023.1 (January 2023)
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