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Literature summary for 1.8.99.5 extracted from

  • Harrison, G.; Curle, C.; Laishley, E.J.
    Purification and characterization of an inducible dissimilatory type sulfite reductase from Clostridium pasteurianum (1984), Arch. Microbiol., 138, 72-78.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
KCN 1 mM, complete inhibition Clostridium pasteurianum
additional information not inhibitory: iodoacetate Clostridium pasteurianum
NaN3 1 mM, 18% inhibition Clostridium pasteurianum
Tris/HCl Tris buffer has an inhibitory effect. Approximately 60% less activity than with phosphate buffer is obtained at pH 7.5 Clostridium pasteurianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
 sulfite pH 7.5, 37°C Clostridium pasteurianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13400
-
 2 * 13400 plus 2 * 28400, SDS-PAGE Clostridium pasteurianum
28400
-
 2 * 13400 plus 2 * 28400, SDS-PAGE Clostridium pasteurianum
83600
-
 PAGE Clostridium pasteurianum

Organism

Organism UniProt Comment Textmining
Clostridium pasteurianum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + a [DsrC protein]-dithiol + 2 reduced acceptor + 2 H+
-
 Clostridium pasteurianum ? + a [DsrC protein]-disulfide + 2 acceptor + 3 H2O 50% of the activity with sulfite ?
additional information no substrates: S3O62-, S2O32-. Methyl viologen is as effective as ferredoxin in coupling the sulfite reductase with hydrogenase while benzyl viologen is only 16% as effective as ferredoxin. No activity is observed with NAD, NADP, FAD or FMN. Varying the substrate concentration [SO 2-] from 1 to 2.5 micromol affects the stoichiometry of the enzyme reaction by alteration of the ratio of H2 uptake to S2- formed from 2.5:1 to 3.1:1 Clostridium pasteurianum ?
-
 ?
nitrite + a [DsrC protein]-dithiol + 2 reduced acceptor
-
 Clostridium pasteurianum ? + a [DsrC protein]-disulfide + 2 acceptor + 2 H2O 25% of the activity with sulfite ?
SeO32- + a [DsrC protein]-dithiol + 2 reduced methyl viologen + 2 H+
-
 Clostridium pasteurianum HSe- + a [DsrC protein]-disulfide + 2 oxidized methyl viologen + 3 H2O 40% of the activity with sulfite ?
sulfite + a [DsrC protein]-dithiol + 2 reduced ferredoxin + 2 H+
-
 Clostridium pasteurianum hydrogen sulfide + a [DsrC protein]-disulfide + 2 oxidized ferredoxin + 3 H2O overall reaction ?

Subunits

Subunits Comment Organism
heterotetramer 2 * 13400 plus 2 * 28400, SDS-PAGE Clostridium pasteurianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 phosphate buffer Clostridium pasteurianum

Cofactor

Cofactor Comment Organism Structure
additional information no activity is observed with NAD, NADP, FAD or FMN Clostridium pasteurianum