General Stability | Organism |
---|---|
total enzyme activity is significantly decreased by ammonium sulfate precipitation, leaving 20% of the initial activity in the supernatant | uncultured Desulfoarculaceae bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
1 * 70000 + 1 * 20000, SDS-PAGE (in denaturing gels, only the small subunit is visible because the large AprA subunit probably overlapped with the highly abundant a-subunit of reverse methylcoenzyme M reductase) | uncultured Desulfoarculaceae bacterium |
70000 | - |
1 * 70000 + 1 * 20000, SDS-PAGE (in denaturing gels, only the small subunit is visible because the large AprA subunit probably overlapped with the highly abundant a-subunit of reverse methylcoenzyme M reductase) | uncultured Desulfoarculaceae bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured Desulfoarculaceae bacterium | F6KEW4 and F6KEW3 | large subunit AprA and small subunit AprB | - |
Purification (Comment) | Organism |
---|---|
partial purification by ammonium sulfate precipitation and Q-Sepharose column chromatography | uncultured Desulfoarculaceae bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + sulfite + [Fe(CN)6]3- | - |
uncultured Desulfoarculaceae bacterium | adenylyl sulfate + [Fe(CN)6]4- | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 70000 + 1 * 20000, SDS-PAGE (in denaturing gels, only the small subunit is visible because the large AprA subunit probably overlapped with the highly abundant a-subunit of reverse methylcoenzyme M reductase) | uncultured Desulfoarculaceae bacterium |
Synonyms | Comment | Organism |
---|---|---|
adenosine-5'-phosphosulfate reductase | - |
uncultured Desulfoarculaceae bacterium |
APR | - |
uncultured Desulfoarculaceae bacterium |
AprB | small subunit | uncultured Desulfoarculaceae bacterium |
APS reductase | - |
uncultured Desulfoarculaceae bacterium |