Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Megalodesulfovibrio gigas |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 20% (w/v) PEG 6000 and 60 mM ammonium sulfate in Tris buffer (0.1 M, pH 7.0), at 18°C | Megalodesulfovibrio gigas |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Megalodesulfovibrio gigas | - |
strain ATCC 19364 | - |
Purification (Comment) | Organism |
---|---|
DE-52 column chromatography, macro-DEAE column chromatography, and hydroxyapatite column chromatography | Megalodesulfovibrio gigas |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + sulfite + ferricyanide | - |
Megalodesulfovibrio gigas | adenylyl sulfate + ferrocyanide | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | APSR comprises six alphabeta-heterodimers that form a hexameric structure, X-ray crystallography | Megalodesulfovibrio gigas |
Synonyms | Comment | Organism |
---|---|---|
adenosine 5'-phosphosulfate reductase | - |
Megalodesulfovibrio gigas |
adenylylsulfate reductase | - |
Megalodesulfovibrio gigas |
APS reductase | - |
Megalodesulfovibrio gigas |
APSR | - |
Megalodesulfovibrio gigas |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FAD is noncovalently attached to the alpha-subunit | Megalodesulfovibrio gigas | |
iron-sulfur centre | two [4Fe-4S] clusters are enveloped by cluster-binding motifs | Megalodesulfovibrio gigas |