Literature summary for 1.8.99.2 extracted from

Chiang, Y.L.; Hsieh, Y.C.; Fang, J.Y.; Liu, E.H.; Huang, Y.C.; Chuankhayan, P.; Jeyaraman, J.; Liu, M.Y.; Chan, S.I.; Chen, C.J.
Crystal structure of adenylylsulfate reductase from Desulfovibrio gigas suggests a potential self-regulation mechanism involving the C-terminus of the beta-subunit (2009), J. Bacteriol., 191, 7597-7608.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli JM109 cells	Megalodesulfovibrio gigas

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 20% (w/v) PEG 6000 and 60 mM ammonium sulfate in Tris buffer (0.1 M, pH 7.0), at 18°C	Megalodesulfovibrio gigas

Organism

Organism	UniProt	Comment	Textmining
Megalodesulfovibrio gigas	-	strain ATCC 19364	-

Purification (Commentary)

Purification (Comment)	Organism
DE-52 column chromatography, macro-DEAE column chromatography, and hydroxyapatite column chromatography	Megalodesulfovibrio gigas

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
AMP + sulfite + ferricyanide	-	Megalodesulfovibrio gigas	adenylyl sulfate + ferrocyanide	-	?

Subunits

Subunits	Comment	Organism
hexamer	APSR comprises six alphabeta-heterodimers that form a hexameric structure, X-ray crystallography	Megalodesulfovibrio gigas

Synonyms

Synonyms	Comment	Organism
adenosine 5'-phosphosulfate reductase	-	Megalodesulfovibrio gigas
adenylylsulfate reductase	-	Megalodesulfovibrio gigas
APS reductase	-	Megalodesulfovibrio gigas
APSR	-	Megalodesulfovibrio gigas

Cofactor

Cofactor	Comment	Organism	Structure
FAD	FAD is noncovalently attached to the alpha-subunit	Megalodesulfovibrio gigas
iron-sulfur centre	two [4Fe-4S] clusters are enveloped by cluster-binding motifs	Megalodesulfovibrio gigas

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Release 2023.1 (January 2023)