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Literature summary for 1.8.99.2 extracted from

  • Berndt, C.; Lillig, C.H.; Wollenberg, M.; Bill, E.; Mansilla, M.C.; de Mendoza, D.; Seidler, A.; Schwenn, J.D.
    Characterization and reconstitution of a 4Fe-4S adenylyl sulfate/phosphoadenylyl sulfate reductase from Bacillus subtilis (2004), J. Biol. Chem., 279, 7850-7855.
    View publication on PubMed
Search for more literature for 1.8.99.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli of a tagged recombinant protein Bacillus subtilis

General Stability

General Stability Organism
unstable to air with a half-life of 12 min, the 4Fe-4S cluster is converted into a 2Fe-2S form Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 reduced glutaredoxin 1 pH 8 and saturating adenylyl sulfate Bacillus subtilis
0.029
-
 adenylyl sulfate pH 8 and saturating thioredoxin 1 Bacillus subtilis
0.053
-
 thioredoxin 1 pH 8 and saturating adenylyl sulfate Bacillus subtilis
0.105
-
 adenylyl sulfate pH 8 and saturating glutaredoxin 1 Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains a cubane 4Fe-4S cluster Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 2 * 27000, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Oxidation Stability

Oxidation Stability Organism
oxygen causes complete loss of activity Bacillus subtilis

Purification (Commentary)

Purification (Comment) Organism
purification of a recombinant tagged protein by chitin affinity chromatography Bacillus subtilis

Renatured (Commentary)

Renatured (Comment) Organism
the inactive 2Fe-2S form can be converted into the 4Fe-4S form by adding an iron-sulfur cluster synthesizing-protein from Synechocystis, restoring the activity Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenylyl sulfate + reduced glutaredoxin 1 can use 3'-phosphoadenylyl sulfate as sulfate donor Bacillus subtilis AMP + sulfite + oxidized glutaredoxin 1
-
 ?
adenylyl sulfate + reduced thioredoxin 1 can use 3'-phosphoadenylyl sulfate as sulfate donor Bacillus subtilis AMP + sulfite + oxidized thioredoxin 1
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 27000, SDS-PAGE Bacillus subtilis