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Literature summary for 1.8.99.2 extracted from

  • Fritz, G.; Buchert, T.; Kroneck, P.M.H.
    The function of the [4Fe-4S] clusters and FAD in bacterial and archaeal adenylylsulfate reductases: evidence for flavin-catalyzed reduction of adenosine 5'-phosphosulfate (2002), J. Biol. Chem., 277, 26066-26073.
    View publication on PubMed
Search for more literature for 1.8.99.2

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
 Desulfovibrio vulgaris
AMP above 0.2 mM Thiobacillus denitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 adenylyl sulfate
-
 Archaeoglobus fulgidus
0.02
-
 AMP
-
 Thiobacillus denitrificans
0.05
-
 AMP
-
 Desulfovibrio vulgaris
0.13
-
 sulfite
-
 Desulfovibrio vulgaris
0.17
-
 AMP
-
 Desulfovibrio desulfuricans
0.18
-
 sulfite
-
 Desulfovibrio desulfuricans
0.2
-
 sulfite
-
 Desulfovibrio vulgaris
0.3
-
 AMP
-
 Desulfovibrio vulgaris
0.34
-
 sulfite
-
 Desulfovibrio vulgaris
1.2
-
 sulfite
-
 Thiobacillus denitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
Iron
-
 Desulfovibrio desulfuricans
Iron
-
 Thiobacillus denitrificans
Iron contains 5.6 iron per alpha,beta heterodimer, novel Fe-S structure, possibly with an iron-nuclearity greater than four Desulfovibrio vulgaris
Iron two [4Fe-4S] clusters, one cluster is deeply buried in the protein matrix, Fe-S center I, center II is located at the surface of the protein Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25600
-
 alpha2beta2, 2 * 67800 + 2 * 25600, smallest catalytic active unit, SDS-PAGE Desulfovibrio vulgaris
67800
-
 alpha2beta2, 2 * 67800 + 2 * 25600, smallest catalytic active unit, SDS-PAGE Desulfovibrio vulgaris
186000
-
 smallest catalytic active subunit, also aggregations of 324000 Da and 569000 Da are detected, gel filtration Desulfovibrio vulgaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenylyl sulfate + reduced acceptor Desulfovibrio desulfuricans key step in sulfur cycle AMP + sulfite + acceptor
-
 ?
adenylyl sulfate + reduced acceptor Thiobacillus denitrificans key step in sulfur cycle AMP + sulfite + acceptor
-
 ?
adenylyl sulfate + reduced acceptor Desulfovibrio vulgaris key step in sulfur cycle AMP + sulfite + acceptor
-
 ?
adenylyl sulfate + reduced acceptor Archaeoglobus fulgidus key step in sulfur cycle AMP + sulfite + acceptor
-
 ?
adenylyl sulfate + reduced acceptor Desulfovibrio vulgaris Hildenborough key step in sulfur cycle AMP + sulfite + acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-
Desulfovibrio desulfuricans
-
-
-
Desulfovibrio vulgaris
-
-
-
Desulfovibrio vulgaris Hildenborough
-
-
-
Thiobacillus denitrificans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Desulfovibrio desulfuricans
-
 Desulfovibrio vulgaris
-
 Archaeoglobus fulgidus

Reaction

Reaction Comment Organism Reaction ID
AMP + sulfite + acceptor = adenylyl sulfate + reduced acceptor mechanism Desulfovibrio desulfuricans
a
AMP + sulfite + acceptor = adenylyl sulfate + reduced acceptor mechanism Thiobacillus denitrificans
a
AMP + sulfite + acceptor = adenylyl sulfate + reduced acceptor mechanism Desulfovibrio vulgaris
a
AMP + sulfite + acceptor = adenylyl sulfate + reduced acceptor mechanism Archaeoglobus fulgidus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenylyl sulfate + dithionite
-
 Archaeoglobus fulgidus AMP + sulfite + ?
-
 ?
adenylyl sulfate + reduced acceptor key step in sulfur cycle Desulfovibrio desulfuricans AMP + sulfite + acceptor
-
 ?
adenylyl sulfate + reduced acceptor key step in sulfur cycle Thiobacillus denitrificans AMP + sulfite + acceptor
-
 ?
adenylyl sulfate + reduced acceptor key step in sulfur cycle Desulfovibrio vulgaris AMP + sulfite + acceptor
-
 ?
adenylyl sulfate + reduced acceptor key step in sulfur cycle Archaeoglobus fulgidus AMP + sulfite + acceptor
-
 ?
adenylyl sulfate + reduced acceptor key step in sulfur cycle Desulfovibrio vulgaris Hildenborough AMP + sulfite + acceptor
-
 ?
adenylyl sulfate + reduced methyl viologen
-
 Desulfovibrio desulfuricans AMP + sulfite + methyl viologen
-
 ?
adenylyl sulfate + reduced methyl viologen
-
 Thiobacillus denitrificans AMP + sulfite + methyl viologen
-
 ?
adenylyl sulfate + reduced methyl viologen
-
 Desulfovibrio vulgaris AMP + sulfite + methyl viologen
-
 ?
adenylyl sulfate + reduced methyl viologen
-
 Archaeoglobus fulgidus AMP + sulfite + methyl viologen
-
 ?
adenylyl sulfate + reduced methyl viologen
-
 Desulfovibrio vulgaris Hildenborough AMP + sulfite + methyl viologen
-
 ?
AMP + sulfite + ferricyanide
-
 Desulfovibrio desulfuricans adenylyl sulfate + ferrocyanide
-
 ?
AMP + sulfite + ferricyanide
-
 Thiobacillus denitrificans adenylyl sulfate + ferrocyanide
-
 ?
AMP + sulfite + ferricyanide
-
 Desulfovibrio vulgaris adenylyl sulfate + ferrocyanide
-
 ?
AMP + sulfite + ferricyanide
-
 Archaeoglobus fulgidus adenylyl sulfate + ferrocyanide
-
 ?
AMP + sulfite + ferricyanide
-
 Desulfovibrio vulgaris Hildenborough adenylyl sulfate + ferrocyanide
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Archaeoglobus fulgidus
tetramer alpha2beta2, 2 * 67800 + 2 * 25600, smallest catalytic active unit, SDS-PAGE Desulfovibrio vulgaris

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Desulfovibrio desulfuricans
FAD
-
 Thiobacillus denitrificans
FAD
-
 Desulfovibrio vulgaris
FAD located nearby Fe-S center I, pointing ist C-8 methyl group to the iron-sulfur center Archaeoglobus fulgidus