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Literature summary for 1.8.99.2 extracted from
- The active centers of adenylylsulfate reductase from Desulfovibrio gigas. Characterization and spectroscopic studies (1990), Eur. J. Biochem., 188, 653-664.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | iron sulfur protein, contains 8 iron atoms per 93000 Da protein, two [4Fe-4S] clusters | Megalodesulfovibrio gigas |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
x * 70000 + x * 26000, SDS-PAGE | Megalodesulfovibrio gigas |
| 70000 | - |
x * 70000 + x * 26000, SDS-PAGE | Megalodesulfovibrio gigas |
| 400000 | - |
gel filtration | Megalodesulfovibrio gigas |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Megalodesulfovibrio gigas | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Megalodesulfovibrio gigas |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Megalodesulfovibrio gigas |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, much loss of activity after 30 days | Megalodesulfovibrio gigas |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenylyl sulfate + dithionite | - |
Megalodesulfovibrio gigas | AMP + sulfite + ? | - |
? | |
| adenylyl sulfate + reduced methyl viologen | - |
Megalodesulfovibrio gigas | AMP + sulfite + methyl viologen | - |
? | |
| AMP + sulfite + ferricyanide | - |
Megalodesulfovibrio gigas | adenylyl sulfate + ferrocyanide | - |
? | |
| CMP + sulfite + ferricyanide | 41% of the activity with AMP | Megalodesulfovibrio gigas | cytidylyl sulfate + ferrocyanide | - |
? | |
| GMP + sulfite + ferricyanide | GMP is as active as AMP | Megalodesulfovibrio gigas | guanylyl sulfate + ferrocyanide | - |
? | |
| UMP + sulfite + ferricyanide | 36% of the activity with AMP | Megalodesulfovibrio gigas | uridylyl sulfate + ferrocyanide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000 + x * 26000, SDS-PAGE | Megalodesulfovibrio gigas |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Megalodesulfovibrio gigas |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | enzyme contains 1 mol of FAD per mol of enzyme | Megalodesulfovibrio gigas | |
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Release 2023.1 (January 2023)
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