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Literature summary for 1.8.98.5 extracted from
- H2 heterodisulfide oxidoreductase complex from Methanobacterium thermoautotrophicum Composition and properties (1994), Eur. J. Biochem., 220, 139-148 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| coenzyme F430 | - |
Methanobacterium thermoautotrophicus |  |
| potassium phosphate | - |
Methanobacterium thermoautotrophicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic membrane | - |
Methanobacterium thermoautotrophicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | the enzyme contains approximately 26 mol non-heme iron | Methanobacterium thermoautotrophicus | |
| Ni2+ | the enzyme contains approximately 0.6 mol nickel | Methanobacterium thermoautotrophicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 250000 | - |
gel filtration | Methanobacterium thermoautotrophicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB | Methanobacterium thermoautotrophicus | - |
reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+ | - |
? | |
| H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB | Methanobacterium thermoautotrophicus DSM 2133 | - |
reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanobacterium thermoautotrophicus | - |
- |
- |
| Methanobacterium thermoautotrophicus DSM 2133 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose column chromatography, phenyl-Sepharose column chromatography, and hydroxyapatite column chromatography | Methanobacterium thermoautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB | - |
Methanobacterium thermoautotrophicus | reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+ | - |
? | |
| H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB | - |
Methanobacterium thermoautotrophicus DSM 2133 | reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+ | - |
? | |
| H2 + oxidized methyl viologen + CoM-S-S-CoB | - |
Methanobacterium thermoautotrophicus | reduced methyl viologen + CoB + CoM + H+ | - |
? | |
| H2 + oxidized methyl viologen + CoM-S-S-CoB | - |
Methanobacterium thermoautotrophicus DSM 2133 | reduced methyl viologen + CoB + CoM + H+ | - |
? | |
| additional information | no activity with coenzyme F420 | Methanobacterium thermoautotrophicus | ? | - |
? | |
| additional information | no activity with coenzyme F420 | Methanobacterium thermoautotrophicus DSM 2133 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterohexamer | 2 * 80000 + 2 * 51000 + 2 * 41000 + 2 * 36000 + 2 * 21000 + 2 * 17000, SDS-PAGE | Methanobacterium thermoautotrophicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| H2: heterodisulfide oxidoreductase complex | - |
Methanobacterium thermoautotrophicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | the enzyme contains approximately 0.9 mol nickel | Methanobacterium thermoautotrophicus | |
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