Activating Compound | Comment | Organism | Structure |
---|---|---|---|
potassium phosphate | complex is most active in presence of 1.6 M potassium phosphate | Methanothermobacter marburgensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Methanothermobacter marburgensis | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter marburgensis | Q50754 | subunit HrdC | - |
Methanothermobacter marburgensis | Q50755 | subunit HrdB | - |
Methanothermobacter marburgensis | Q50756 | subunit HrdA | - |
Methanothermobacter marburgensis DSM 2133 | Q50754 | subunit HrdC | - |
Methanothermobacter marburgensis DSM 2133 | Q50755 | subunit HrdB | - |
Methanothermobacter marburgensis DSM 2133 | Q50756 | subunit HrdA | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
110 | - |
HdrABC complex with 100% H2 in the gas phase, pH 7.6, 60°C | Methanothermobacter marburgensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coenzyme B-coenzyme M disulfide + ferredoxin + 2 H2 | HdrABC catalyzes the CoM-S-S-CoB-dependent reduction of ferredoxin with H2, coupling the endergonic reduction of ferredoxin to the exergonic reduction of coenzyme B-coenzyme M disulfide. Per mole CoM-S-S-CoB added, 1 mol of ferredoxin is reduced, indicating an electron bifurcation coupling mechanism. The stoichiometry of coupling is consistent with an ATP gain per mole methane from 4 H2 and CO2 of near 0.5 | Methanothermobacter marburgensis | coenzyme B + coenzyme M + reduced ferredoxin2- + 2 H+ | - |
? | |
coenzyme B-coenzyme M disulfide + ferredoxin + 2 H2 | HdrABC catalyzes the CoM-S-S-CoB-dependent reduction of ferredoxin with H2, coupling the endergonic reduction of ferredoxin to the exergonic reduction of coenzyme B-coenzyme M disulfide. Per mole CoM-S-S-CoB added, 1 mol of ferredoxin is reduced, indicating an electron bifurcation coupling mechanism. The stoichiometry of coupling is consistent with an ATP gain per mole methane from 4 H2 and CO2 of near 0.5 | Methanothermobacter marburgensis DSM 2133 | coenzyme B + coenzyme M + reduced ferredoxin2- + 2 H+ | - |
? | |
coenzyme B-coenzyme M disulfide + metronidazole + 2 H2 | purified complex shows a twofold higher specific activity with ferredoxin than with metronidazole | Methanothermobacter marburgensis | coenzyme B + coenzyme M + reduced metronidazole2- + 2 H+ | - |
? | |
coenzyme B-coenzyme M disulfide + metronidazole + 2 H2 | purified complex shows a twofold higher specific activity with ferredoxin than with metronidazole | Methanothermobacter marburgensis DSM 2133 | coenzyme B + coenzyme M + reduced metronidazole2- + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HdrA | - |
Methanothermobacter marburgensis |
HdrABC | - |
Methanothermobacter marburgensis |
HdrB | - |
Methanothermobacter marburgensis |
HdrC | - |
Methanothermobacter marburgensis |
MvhADG | - |
Methanothermobacter marburgensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Methanothermobacter marburgensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Methanothermobacter marburgensis |