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Literature summary for 1.8.98.1 extracted from
- Purification and properties of the heme- and iron-sulfur-containing heterodisulfide reductase from Methanosarcina thermophila (1998), Biochemistry, 37, 10027-10039.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Methanosarcina thermophila | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | the enzyme contains two distinct [Fe4S4]2+/1+ clusters in the large subunit | Methanosarcina thermophila |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
x * 53000 + x * 27000, SDS-PAGE | Methanosarcina thermophila |
| 53000 | - |
x * 53000 + x * 27000, SDS-PAGE | Methanosarcina thermophila |
| 155000 | - |
gel filtration | Methanosarcina thermophila |
| 206000 | - |
equilibrium sedimentation | Methanosarcina thermophila |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coenzyme B + coenzyme M + methanophenazine | Methanosarcina thermophila | - |
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina thermophila | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| lipoprotein | - |
Methanosarcina thermophila |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanosarcina thermophila |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:acetate-grown cell | - |
Methanosarcina thermophila | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 200 | - |
- |
Methanosarcina thermophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coenzyme B + coenzyme M + methanophenazine | - |
Methanosarcina thermophila | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine | - |
? | |
| N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen | - |
Methanosarcina thermophila | coenzyme B + coenzyme M + methylviologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000 + x * 27000, SDS-PAGE | Methanosarcina thermophila |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HDR | - |
Methanosarcina thermophila |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | the enzyme contains two discrete b-type hemes in the small subunit. One heme is high-spin and has a high midpoint potential, -23 mV, whereas the other heme is low-spin and exhibits a relatively low midpoint potential, -180 mV | Methanosarcina thermophila | |
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