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Literature summary for 1.8.98.1 extracted from
- Purification and properties of heterodisulfide reductase from Methanobacterium thermoautotrophicum (strain Marburg) (1990), Eur. J. Biochem., 193, 255-261.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (2R,3R)-3-hydroxy-2-([6-[(2-sulfoethyl)dithio]hexanoyl]amino)butanoic acid | Ki above 2 mM | Methanothermobacter thermautotrophicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the KM-value for coenzyme B is below 0.05 mM at pH 7.6, 65°C | Methanothermobacter thermautotrophicus | |
| additional information | - |
additional information | the KM-value for reduced benzyl viologen is below 0.05 mM | Methanothermobacter thermautotrophicus | |
| 0.1 | - |
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine | pH 7.6, 54°C | Methanothermobacter thermautotrophicus | |
| 0.13 | - |
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine | pH 7.6, 65°C | Methanothermobacter thermautotrophicus | |
| 0.2 | - |
coenzyme M | pH 7.6, 65°C | Methanothermobacter thermautotrophicus | |
| 0.2 | - |
6-Mercaptohexanoyl-L-threonine phosphate | pH 7.6, 54°C | Methanothermobacter thermautotrophicus | |
| 3 | - |
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-D-threonine | pH 7.6, 65°C | Methanothermobacter thermautotrophicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
alpha4beta4gamma4, x * 80000 + x * 36000 + x * 21000, the native enzyme is probably a tetramer of trimers | Methanothermobacter thermautotrophicus |
| 36000 | - |
alpha4beta4gamma4, x * 80000 + x * 36000 + x * 21000, the native enzyme is probably a tetramer of trimers | Methanothermobacter thermautotrophicus |
| 80000 | - |
alpha4beta4gamma4, x * 80000 + x * 36000 + x * 21000, the native enzyme is probably a tetramer of trimers | Methanothermobacter thermautotrophicus |
| 350000 | 400000 | gel filtration | Methanothermobacter thermautotrophicus |
| 550000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coenzyme B + coenzyme M + methanophenazine | Methanothermobacter thermautotrophicus | - |
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine | - |
? | |
| coenzyme B + coenzyme M + methanophenazine | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | - |
- |
- |
| Methanothermobacter thermautotrophicus | - |
- |
- |
| Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| partially purified enzyme is insensitive towards O2 | Methanothermobacter thermautotrophicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanothermobacter thermautotrophicus |
| partial | Methanothermobacter thermautotrophicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.6 | - |
formation of the heterodisulfide, pH 7.6, 40°C | Methanosarcina barkeri |
| 0.62 | - |
reduction of the heterodisulfide, pH 7.6, 40°C | Methanosarcina barkeri |
| 10 | - |
- |
Methanothermobacter thermautotrophicus |
| 29 | - |
- |
Methanothermobacter thermautotrophicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, more than 80% inactivation within 12 h under aerobic conditions | Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coenzyme B + 6-mercaptohexanoyl-L-threonine phosphate + methylene blue | - |
Methanothermobacter thermautotrophicus | ? + methylene blue | - |
ir | |
| coenzyme B + 6-mercaptohexanoyl-L-threonine phosphate + methylene blue | - |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | ? + methylene blue | - |
ir | |
| coenzyme B + coenzyme M + methanophenazine | - |
Methanothermobacter thermautotrophicus | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine | - |
? | |
| coenzyme B + coenzyme M + methanophenazine | - |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine | - |
? | |
| additional information | homodisulfides of coenzyme M and coenzyme B are not reduced | Methanothermobacter thermautotrophicus | ? | - |
? | |
| additional information | the enzyme does not catalyze the reduction of N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine with NADH, NADPH or reduced coenzyme F420 | Methanothermobacter thermautotrophicus | ? | - |
? | |
| additional information | homodisulfides of coenzyme M and coenzyme B are not reduced | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | ? | - |
? | |
| additional information | the enzyme does not catalyze the reduction of N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine with NADH, NADPH or reduced coenzyme F420 | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | ? | - |
? | |
| N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | the D-enantiomer of the heterodisulfide is reduced at approximately 35% of the rate observed for the L-form | Methanothermobacter thermautotrophicus | coenzyme B + coenzyme M | - |
? | |
| N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | the D-enantiomer of the heterodisulfide is reduced at approximately 35% of the rate observed for the L-form | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | coenzyme B + coenzyme M | - |
? | |
| N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced benzylviologen | - |
Methanothermobacter thermautotrophicus | coenzyme B + coenzyme M + benzylviologen | - |
? | |
| N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced benzylviologen | - |
Methanothermobacter thermautotrophicus | coenzyme B + coenzyme M + benzylviologen | - |
r | |
| N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylene blue | - |
Methanosarcina barkeri | coenzyme B + coenzyme M + methylene blue | - |
r | |
| N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylene blue | - |
Methanothermobacter thermautotrophicus | coenzyme B + coenzyme M + methylene blue | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | alpha4beta4gamma4, x * 80000 + x * 36000 + x * 21000, the native enzyme is probably a tetramer of trimers | Methanothermobacter thermautotrophicus |
| More | four major protein bands of 40000 Da, 50000 Da, 55000 Da and 66000 Da are detected by SDS-PAGE | Methanothermobacter thermautotrophicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CoM-S-S-HTP reductase | - |
Methanothermobacter thermautotrophicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 80 | - |
Methanothermobacter thermautotrophicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
30 min, 50% loss of activity | Methanothermobacter thermautotrophicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Methanothermobacter thermautotrophicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | enzyme contains 4 mol FAD per mol of native enzyme | Methanothermobacter thermautotrophicus | |
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