Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.7.2 extracted from

  • Chow, L.P.; Iwadate, H.; Yano, K.; Kamo, M.; Tsugita, A.; Gardet-Salvi, L.; Stritt-Etter, A.L.; Schurmann, P.
    Amino acid sequence of spinach ferredoxin:thioredoxin reductase catalytic subunit and identification of thiol groups constituting a redox-active disulfide and a [4Fe-4S] cluster (1995), Eur. J. Biochem., 231, 149-156.
    View publication on PubMed
Search for more literature for 1.8.7.2

Protein Variants

Protein Variants Comment Organism
additional information modification of the redox-active disulfide in the active site and of other cysteinyl residues, FTR is incubated for 20 rnin at 25°C with 2 mM N-ethylmaleimide or [l4C]N-ethylmaleimide in 100 mM sodium phosphate, pH 7.0, for the modification of the accessible cysteines and additionally in the presence of 2% SDS for the inaccessible cysteines Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Spinacia oleracea 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ ferredoxin:thioredoxin reductase is a [4Fe-4S] protein Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12959
-
 x * ? + x * 12959, catalytic subunit, calculated Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-
Spinacia oleracea P41348 B-subunit
-

Subunits

Subunits Comment Organism
? x * ? + x * 12959, catalytic subunit, calculated Spinacia oleracea
dimer alpha/beta, peptide mapping for localization of labeled cysteinyl residues, overview Spinacia oleracea
More amino acid sequence of the catalytic subunit. Six of the eight cysteine residues are clustered as Cys-Pro-Cys and Cys-His-Cys groups. Cysl9 and Cys27 are free cysteines with no catalytic function, Cys54 and Cys84 constitute the redox-active disulfide bridge of the active site, and the remaining four, Cys52, Cys71, Cys73, and Cys82 bind the Fe-S cluster Spinacia oleracea

Synonyms

Synonyms Comment Organism
FTR
-
 Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
4Fe-4S-center residues Cys52, Cys71, Cys73, and Cys82 bind the Fe-S cluster Spinacia oleracea

pI Value

Organism Comment pI Value Maximum pI Value
Spinacia oleracea catalytic subunit, calculated
-
 5.3

General Information

General Information Comment Organism
physiological function ferredoxin:thioredoxin reductase is the key enzyme of the ferredoxid-thioredoxin system, and is involved in the light regulation of carbon metabolism in oxygenic photosynthesis catalyzing the reduction of thioredoxins with light-generated electrons Spinacia oleracea