Any feedback?
Literature summary for 1.8.7.1 extracted from
- Siroheme- and [Fe4-S4]-dependent NirA from Mycobacterium tuberculosis is a sulfite reductase with a covalent Cys-Tyr bond in the active site (2005), J. Biol. Chem., 280, 27319-27328.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | potential target for the development of antituberculosis agents | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapour diffusion method using 0.1 M Tris-HCl, pH range 8.2-8.7, 0.2 M MgCl2, and 30% polyethylene glycol 4000 | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C161A | catalytically impaired mutant, tested in an assay using the nonphysiological electron donor methyl viologen and sulfite as substrate | Mycobacterium tuberculosis |
| C161S | catalytically impaired mutant, tested in an assay using the nonphysiological electron donor methyl viologen and sulfite as substrate | Mycobacterium tuberculosis |
| Y69A | catalytically impaired mutant, tested in an assay using the nonphysiological electron donor methyl viologen and sulfite as substrate | Mycobacterium tuberculosis |
| Y69F | catalytically impaired mutant, tested in an assay using the nonphysiological electron donor methyl viologen and sulfite as substrate | Mycobacterium tuberculosis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| [4Fe-4S] center | - |
Mycobacterium tuberculosis |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WJ03 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WJ03 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-nitrilotriacetic acid affinity matrix and Q-Sepharose column chromatography | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme has a specific activity of 2.0 units/mg protein | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | NirA has a pronounced preference for sulfite over nitrite as substrate | Mycobacterium tuberculosis | ? | - |
? | |
| additional information | NirA has a pronounced preference for sulfite over nitrite as substrate | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| sulfite + methyl viologen | - |
Mycobacterium tuberculosis | sulfide + ? | - |
? | |
| sulfite + methyl viologen | - |
Mycobacterium tuberculosis H37Rv | sulfide + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | x-ray crystallography | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ferredoxin-dependent sulfite reductase | - |
Mycobacterium tuberculosis |
| NirA | - |
Mycobacterium tuberculosis |
| siroheme- and [Fe4-S4]-dependent NirA | - |
Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| siroheme | - |
Mycobacterium tuberculosis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
