Literature summary for 1.8.5.B1 extracted from

Juillan-Binard, C.; Picciocchi, A.; Andrieu, J.P.; Dupuy, J.; Petit-Hartlein, I.; Caux-Thang, C.; Vives, C.; Niviere, V.; Fieschi, F.
A two-component NADPH oxidase (NOX)-like system in bacteria is involved in the electron transfer chain to the methionine sulfoxide reductase MsrP (2017), J. Biol. Chem., 292, 2485-2494 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
H151A	mutant has aout 57% of the heme content of wild-type	Escherichia coli
H164A	mutant has aout 63% of the heme content of wild-type	Escherichia coli
H91A	almost complete loss of heme binding	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	subunit MsrQ	Escherichia coli	16020	-

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P76342 and P76343	P76342 i.e. catalytic subunit MsrP, P76343 i.e. heme-binding subunit MsrQ	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	subunit MsrQ is reduced due to the free reduced FMN generated by the flavin reductase Fre activity	Escherichia coli	?	-	?
[protein]-L-methionine + a quinone + H2O	-	Escherichia coli	[protein]-L-methionine (R)-S-oxide + a quinol	-	r
[protein]-L-methionine + a quinone + H2O	-	Escherichia coli	[protein]-L-methionine (S)-S-oxide + a quinol	-	r

Subunits

Subunits	Comment	Organism
More	protein physically interacts with flavin reductase Fre	Escherichia coli

Synonyms

Synonyms	Comment	Organism
MsrP	-	Escherichia coli
MsrQ	-	Escherichia coli
protein-methionine-sulfoxide reductase	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
heme	heme/protein ratio is 2.5. The extinction coefficient value for the oxidized subunit MsrQ form at 412 nm is 51950 per M and cm. For the reduced MsrQ form at 426 and 558 nm, the extinction coefficient values are 69015 per M and cm and 11075 per M and cm, respectively	Escherichia coli

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Release 2023.1 (January 2023)