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Literature summary for 1.8.5.4 extracted from
- Role of human sulfide quinone oxidoreductase in H2S metabolism (2015), Methods Enzymol., 554, 255-270 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | 4.5fold increase in the rate of H2S oxidation in the presence of 1 mM cyanide | Homo sapiens |  |
| sulfite | 13.6fold increase in the rate of H2S oxidation in the presence of 0.6 mM sulfite | Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0109 | - |
Sulfide | with coenzyme Q and cyanide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
| 0.013 | - |
Sulfide | with coenzyme Q and sulfite as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
| 0.014 | - |
coenzyme Q | with cyanide and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
| 0.019 | - |
coenzyme Q | with sulfite and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
| 0.174 | - |
sulfite | with coenzyme Q and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
| 0.315 | - |
Sulfide | with coenzyme Q as cosubstrate, at pH 8.0 and 25°C | Homo sapiens | |
| 0.65 | - |
cyanide | with coenzyme Q and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial membrane | - |
Homo sapiens | 31966 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfide + coenzyme Q | Homo sapiens | - |
sulfane sulfur + reduced coenzyme Q | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9Y6N5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap IMAC column chromatography and Q Sepharose column chromatography | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.17 | - |
unpurified enzyme, at pH 8.0 and 25°C | Homo sapiens |
| 580.8 | - |
purified enzyme, at pH 8.0 and 25°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfide + coenzyme Q | - |
Homo sapiens | sulfane sulfur + reduced coenzyme Q | - |
? | |
| sulfide + cyanide + coenzyme Q | - |
Homo sapiens | thiocyanate + reduced coenzyme Q | - |
? | |
| sulfide + sulfide + coenzyme Q | - |
Homo sapiens | hydrogen disulfide + reduced coenzyme Q | - |
? | |
| sulfide + sulfite + coenzyme Q | - |
Homo sapiens | thiosulfate + reduced coenzyme Q | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 47000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SQOR | - |
Homo sapiens |
| sulfide: quinone oxidoreductase | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18.5 | - |
Sulfide | with coenzyme Q as cosubstrate, at pH 8.0 and 25°C | Homo sapiens | |
| 65 | - |
Sulfide | with coenzyme Q as cosubstrate, at pH 8.0 and 25°C | Homo sapiens | |
| 330 | - |
cyanide | with coenzyme Q and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
| 343 | - |
Sulfide | with coenzyme Q and cyanide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
| 360 | - |
coenzyme Q | with cyanide and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
| 364 | - |
coenzyme Q | with sulfite and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
| 368 | - |
sulfite | with coenzyme Q and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
| 379 | - |
Sulfide | with coenzyme Q and sulfite as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | the enzyme contains 0.82 mol FAD per mol protein | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 210 | - |
Sulfide | with coenzyme Q as cosubstrate, at pH 8.0 and 25°C | Homo sapiens | |
| 510 | - |
cyanide | with coenzyme Q and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
| 2100 | - |
sulfite | with coenzyme Q and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
| 19000 | - |
coenzyme Q | with sulfite and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
| 27000 | - |
coenzyme Q | with cyanide and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
| 29000 | - |
Sulfide | with coenzyme Q and sulfite as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
| 31000 | - |
Sulfide | with coenzyme Q and cyanide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
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