Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cyanide | 4.5fold increase in the rate of H2S oxidation in the presence of 1 mM cyanide | Homo sapiens | |
sulfite | 13.6fold increase in the rate of H2S oxidation in the presence of 0.6 mM sulfite | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0109 | - |
Sulfide | with coenzyme Q and cyanide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
0.013 | - |
Sulfide | with coenzyme Q and sulfite as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
0.014 | - |
coenzyme Q | with cyanide and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
0.019 | - |
coenzyme Q | with sulfite and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
0.174 | - |
sulfite | with coenzyme Q and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
0.315 | - |
Sulfide | with coenzyme Q as cosubstrate, at pH 8.0 and 25°C | Homo sapiens | |
0.65 | - |
cyanide | with coenzyme Q and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial membrane | - |
Homo sapiens | 31966 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfide + coenzyme Q | Homo sapiens | - |
sulfane sulfur + reduced coenzyme Q | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9Y6N5 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap IMAC column chromatography and Q Sepharose column chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.17 | - |
unpurified enzyme, at pH 8.0 and 25°C | Homo sapiens |
580.8 | - |
purified enzyme, at pH 8.0 and 25°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfide + coenzyme Q | - |
Homo sapiens | sulfane sulfur + reduced coenzyme Q | - |
? | |
sulfide + cyanide + coenzyme Q | - |
Homo sapiens | thiocyanate + reduced coenzyme Q | - |
? | |
sulfide + sulfide + coenzyme Q | - |
Homo sapiens | hydrogen disulfide + reduced coenzyme Q | - |
? | |
sulfide + sulfite + coenzyme Q | - |
Homo sapiens | thiosulfate + reduced coenzyme Q | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 47000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
SQOR | - |
Homo sapiens |
sulfide: quinone oxidoreductase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.5 | - |
Sulfide | with coenzyme Q as cosubstrate, at pH 8.0 and 25°C | Homo sapiens | |
65 | - |
Sulfide | with coenzyme Q as cosubstrate, at pH 8.0 and 25°C | Homo sapiens | |
330 | - |
cyanide | with coenzyme Q and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
343 | - |
Sulfide | with coenzyme Q and cyanide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
360 | - |
coenzyme Q | with cyanide and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
364 | - |
coenzyme Q | with sulfite and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
368 | - |
sulfite | with coenzyme Q and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
379 | - |
Sulfide | with coenzyme Q and sulfite as cosubstrates, at pH 8.0 and 25°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains 0.82 mol FAD per mol protein | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
210 | - |
Sulfide | with coenzyme Q as cosubstrate, at pH 8.0 and 25°C | Homo sapiens | |
510 | - |
cyanide | with coenzyme Q and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
2100 | - |
sulfite | with coenzyme Q and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
19000 | - |
coenzyme Q | with sulfite and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
27000 | - |
coenzyme Q | with cyanide and sulfide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
29000 | - |
Sulfide | with coenzyme Q and sulfite as cosubstrates, at pH 8.0 and 25°C | Homo sapiens | |
31000 | - |
Sulfide | with coenzyme Q and cyanide as cosubstrates, at pH 8.0 and 25°C | Homo sapiens |