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Literature summary for 1.8.5.4 extracted from

  • Shuman, K.; Hanson, T.
    A sulfide quinone oxidoreductase from Chlorobaculum tepidum displays unusual kinetic properties (2016), FEMS Microbiol. Lett., 363, 1-8 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.5.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) pLysScells Chlorobaculum tepidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.95
-
 Sulfide recombinant enzyme, at pH 7.4 and 47°C Chlorobaculum tepidum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43500
-
 gel filtration Chlorobaculum tepidum

Organism

Organism UniProt Comment Textmining
Chlorobaculum tepidum Q8KDG3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Chlorobaculum tepidum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.7
-
 purified recombinant enzyme, at pH 7.4 and 47°C Chlorobaculum tepidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfide + decylubiquinone
-
 Chlorobaculum tepidum sulfur + decylubiquinol
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45800, calculated from amino acid sequence Chlorobaculum tepidum

Synonyms

Synonyms Comment Organism
CT1087
-
 Chlorobaculum tepidum
SQR
-
 Chlorobaculum tepidum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
54
-
 Sulfide recombinant enzyme, at pH 7.4 and 47°C Chlorobaculum tepidum

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Chlorobaculum tepidum