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Literature summary for 1.8.5.3 extracted from
- An active site tyrosine influences the ability of the dimethyl sulfoxide reductase family of molybdopterin enzymes to reduce S-oxides (2001), J. Biol. Chem., 276, 13178-13185.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Cereibacter sphaeroides |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y114A | mutation in direction of the active site of trimethylamine-N-oxide reduxtase. Mutation results in decreased specificity for S-oxides and an increased specificity for trimethylamine-N-oxide | Cereibacter sphaeroides |
| Y114F | mutation in direction of the active site of tiomethylamine-N-oxide reduxtase. Mutation results in decreased specificity for S-oxides and an increased specificity for trimethylamine-N-oxide | Cereibacter sphaeroides |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
Dimethylsulfoxide | wild-type, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides |  |
| 0.02 | - |
adenosine-1N-oxide | wild-type, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 0.08 | - |
adenosine-1N-oxide | mutant Y114A, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 0.18 | - |
Dimethylsulfoxide | mutant Y114F, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 0.33 | - |
methionine sulfoxide | wild-type, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 0.4 | - |
Dimethylsulfoxide | mutant Y114A, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 0.52 | - |
adenosine-1N-oxide | mutant Y114F, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 2.3 | - |
Trimethylamine N-oxide | mutant Y114A, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 16 | - |
methionine sulfoxide | mutant Y114A, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 19 | - |
methionine sulfoxide | mutant Y114F, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 68 | - |
Trimethylamine N-oxide | wild-type, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 88 | - |
Trimethylamine N-oxide | mutant Y114F, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine-1N-oxide + reduced dichlorophenolindophenol | - |
Cereibacter sphaeroides | adenine + H2O + oxidized dichlorophenolindophenol | - |
r | |
| dimethylsulfoxide + reduced dichlorophenolindophenol | - |
Cereibacter sphaeroides | dimethylsulfide + H2O + oxidized dichlorophenolindophenol | - |
r | |
| methionine sulfoxide + reduced dichlorophenolindophenol | - |
Cereibacter sphaeroides | methionine + H2O + oxidized dichlorophenolindophenol | - |
r | |
| trimethylamine N-oxide + reduced dichlorophenolindophenol | - |
Cereibacter sphaeroides | trimethylamine + H2O + oxidized dichlorophenolindophenol | - |
r | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50 | - |
Dimethylsulfoxide | wild-type, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 58 | - |
methionine sulfoxide | wild-type, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 67 | - |
Dimethylsulfoxide | mutant Y114A, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 92 | - |
methionine sulfoxide | mutant Y114A, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 110 | - |
adenosine-1N-oxide | wild-type, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 180 | - |
Dimethylsulfoxide | mutant Y114F, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 180 | - |
methionine sulfoxide | mutant Y114F, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 1200 | - |
adenosine-1N-oxide | mutant Y114A, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 1900 | - |
Trimethylamine N-oxide | mutant Y114A, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 2200 | - |
adenosine-1N-oxide | mutant Y114F, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 2300 | - |
Trimethylamine N-oxide | wild-type, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 4300 | - |
Trimethylamine N-oxide | mutant Y114F, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.8 | - |
methionine sulfoxide | mutant Y114A, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 9.5 | - |
methionine sulfoxide | mutant Y114F, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 34 | - |
Trimethylamine N-oxide | wild-type, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 49 | - |
Trimethylamine N-oxide | mutant Y114F, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 170 | - |
Dimethylsulfoxide | mutant Y114A, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 180 | - |
methionine sulfoxide | wild-type, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 830 | - |
Trimethylamine N-oxide | mutant Y114A, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 1000 | - |
Dimethylsulfoxide | mutant Y114F, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 4200 | - |
adenosine-1N-oxide | mutant Y114F, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 5500 | - |
adenosine-1N-oxide | wild-type, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 7100 | - |
Dimethylsulfoxide | wild-type, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
| 15000 | - |
adenosine-1N-oxide | mutant Y114A, pH 8.0, temperature not specified in the publication | Cereibacter sphaeroides | |
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