Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sodium sulfate | the purified enzyme requires 150 mM sodium sulfate to generate maximum activity | Acidithiobacillus ferrooxidans | |
sulfite | the addition of sulfite (2 mM) results in the activation of the enzyme at a magnitude of 1.6fold | Acidithiobacillus ferrooxidans |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Acidithiobacillus ferrooxidans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Sodium sulfate | 60% reduction in enzyme activity is observed in the reaction mixture containing 200 mM sodium sulfate | Acidithiobacillus ferrooxidans | |
sulfite | almost complete inhibition in the presence of 10 mM sulfite | Acidithiobacillus ferrooxidans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
thiosulfate | recombinant enzyme, at pH 2.5 and 50°C | Acidithiobacillus ferrooxidans | |
15 | - |
thiosulfate | native enzyme, at pH 2.5 and 70°C | Acidithiobacillus ferrooxidans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, SDS-PAGE | Acidithiobacillus ferrooxidans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, CM-650M column chromatography, butyl-650M column chromatography, and TSKgel G3000 gel filtration | Acidithiobacillus ferrooxidans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:sulfur-grown cell | - |
Acidithiobacillus ferrooxidans | - |
culture condition:tetrathionate-grown cell | - |
Acidithiobacillus ferrooxidans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.05 | - |
unpurified recombinant enzyme, at pH 2.5 and 40°C | Acidithiobacillus ferrooxidans |
3.16 | - |
recombinant enzyme after 63.2fold purification, at pH 2.5 and 40°C | Acidithiobacillus ferrooxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme reduces neither ubiquinone nor horse heart cytochrome c, which serves as an electron acceptor | Acidithiobacillus ferrooxidans | ? | - |
? | |
thiosulfate + ferricyanide + H+ | when the concentration of thiosulfate is lower than 4 mM, no enzyme activity is detected | Acidithiobacillus ferrooxidans | tetrathionate + ferrocyanide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Acidithiobacillus ferrooxidans |
Synonyms | Comment | Organism |
---|---|---|
tetrathionate-forming thiosulfate dehydrogenase | - |
Acidithiobacillus ferrooxidans |
TSD | - |
Acidithiobacillus ferrooxidans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Acidithiobacillus ferrooxidans |
70 | - |
native enzyme | Acidithiobacillus ferrooxidans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
native and recombinant enzyme | Acidithiobacillus ferrooxidans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 3 | the native enzyme shows about 35% activity at pH 2.0 and 20% activity at pH 3.0, the recombinant enzyme shows about 80% activity at pH 2.0 and about 35% activity at pH 3.0 | Acidithiobacillus ferrooxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | does not contain heme | Acidithiobacillus ferrooxidans |