Cloned (Comment) | Organism |
---|---|
gene APR1, expression of the holoenzyme APR1p in Escherichia coli strain BL21(DE3), separate expression in Escherichia coli strain BL21(DE3) of amino acid residues 73-327, forming the R-domain, and of residues 328-465, forming the C-domain, the domains alone are inactive, but mixing of both can partially restore activity | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | gene APR1, the holoenzyme APR1p as well as the C-domain alone can complement the cysteine auxotrophy of an Escherichia coli cysH mutant strain, substituting for glutaredoxin, if the mutant is capable of producing glutathione, cysH encodes the 5'-phosphoadenylylsulfate reductase in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutathione | slightly inhibitory at high concentrations | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
glutathione | holoenzyme APR1p with substrate 5'-adenylyl sulfate, pH 8.5, 30°C | Arabidopsis thaliana | |
1.12 | - |
glutathione | about, holoenzyme APR1p and C-domain with substrates hydroxyethyldisulfide, or cystine, pH 8.0, 24°C | Arabidopsis thaliana | |
1.15 | - |
glutathione | about, holoenzyme APR1p and C-domain with substrate dehydroascorbate, pH 6.9, 24°C | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | enzyme is involved in sulfate assimilation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P92979 | APR1; 3 genes APR1, APR2, and APR3, 3 isozymes APR1p, APR2p, and APR3p | - |
Purification (Comment) | Organism |
---|---|
recombinant holoenzyme APR1p, C-domain, and R-domain from Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-adenylyl sulfate + glutathione | catalyzed by the holoenzyme APR1p, not by the C-domain | Arabidopsis thaliana | AMP + sulfite + glutathione disulfide | - |
? | |
cystine + glutathione | catalyzed by the holoenzyme APR1p and the C-domain | Arabidopsis thaliana | cysteine + glutathione disulfide | - |
? | |
dehydroascorbate + glutathione | catalyzed by the holoenzyme APR1p and the C-domain | Arabidopsis thaliana | ascorbate + glutathione disulfide | - |
? | |
hydroxyethyldisulfide + glutathione | catalyzed by the holoenzyme APR1p and the C-domain | Arabidopsis thaliana | ? | - |
? | |
insulin disulfide + glutathione | catalyzed by the C-domain, not by the holoenzyme APR1p or the R-domain | Arabidopsis thaliana | insulin + glutathione disulfide | - |
? | |
additional information | enzyme is involved in sulfate assimilation | Arabidopsis thaliana | ? | - |
? | |
oxidized ribonucleotide reductase + glutathione | catalyzed by the C-domain, not by the holoenzyme APR1p or the R-domain | Arabidopsis thaliana | reduced ribonucleotide reductase + glutathione disulfide | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | amino acid residues 73-327 form the R-domain which is homologous to microbial 5'-phosphoadenylylsulfate reductase, and of residues 328-465 form the C-domain which is homologous to thioredoxin, the domains alone are inactive, but mixing of both can partially rstore activity | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
5'-adenylylsulfate reductase | - |
Arabidopsis thaliana |
APR1p | - |
Arabidopsis thaliana |
APS reductase | - |
Arabidopsis thaliana |
More | enzyme belongs to the thioredoxin superfamily | Arabidopsis thaliana |
plant-type 5'-adenylylsulfate reductase | - |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | 30 | - |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glutathione | dependent on, specific cofactor for the carboxyterminal glutathione-dependent reductase domain of the enzyme, can be exchanged for equally active DTT, glutathione is slightly inhibitory at high concentrations | Arabidopsis thaliana | |
additional information | DTT can be exchanged for glutathione, equally active | Arabidopsis thaliana | |
additional information | no activity with 2-mercaptoethanol, gamma-glutamylcysteine, or cysteine | Arabidopsis thaliana |