Cloned (Comment) | Organism |
---|---|
- |
Lemna minor |
General Stability | Organism |
---|---|
enzyme unstable during purification and storage, addition of 5 mM 5'-adenosine monophosphate stabilizes activity | Lemna minor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-adenylylsulfate | at 0.12 mM | Lemna minor | |
adenosine-5'-phosphate | competitive inhibition, Ki of 0.34 mM with 10 mM glutathione | Lemna minor | |
glutathione | at 60 mM | Lemna minor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
5'-adenylylsulfate | with 10 mM glutathione as reductant | Lemna minor | |
0.0122 | - |
5'-adenylylsulfate | with 40 mM glutathione as reductant | Lemna minor | |
16.8 | - |
glutathione | with 0.04 mM adenylylsulfate | Lemna minor |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | sequence contains chloroplast transit peptide | Lemna minor | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
SDS-PAGE | Lemna minor |
43640 | - |
calculated from cDNA sequence | Lemna minor |
91300 | - |
estimation by gel filtration with Superose 12 | Lemna minor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lemna minor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification to homogeneity | Lemna minor |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | 0.025 | crude extract, plants treated with 10 mM NH4Cl | Lemna minor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenosine-5'-phosphosulfate + glutathione | no activity with 3'-phosphoadenosine-5'-phosphosulfate | Lemna minor | adenosine 3',5'-diphosphate + sulfite + oxidized glutathione | - |
? | |
5'-adenylylsulfate + dithioerythritol | - |
Lemna minor | adenosine 5'-monophosphate + sulfite + oxidized dithioerythritol | - |
? | |
5'-adenylylsulfate + glutathione | - |
Lemna minor | adenosine monophosphate + sulfite + oxidized glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha2, 2 * 43000, comparison of native mass after gel filtration with denatured after SDS-PAGE | Lemna minor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
56 | - |
5'-adenylylsulfate | - |
Lemna minor |