General Stability | Organism |
---|---|
ethylene glycol, 10%, stabilizes | Saccharomyces cerevisiae |
glutathione stabilizes, stabilizing effect increased by cystine | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell associated | - |
Saccharomyces cerevisiae | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cystine + GSH | Saccharomyces cerevisiae | - |
cysteine + GSSG | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
61.6 | - |
beta-hydroxyethyldisulfide | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, pH 5.8, 1 mM glutathione, several months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cystine + GSH | - |
Saccharomyces cerevisiae | cysteine + GSSG | - |
? | |
additional information | inactive with: insulin and other proteins | Saccharomyces cerevisiae | ? | - |
? | |
additional information | at low substrate concentration the reaction rate is greater with L-cystine than with any of the other substances | Saccharomyces cerevisiae | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
54 | - |
crude state, quite labile to heat above 54°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
pH 7.8 chosen for the assay to achieve smaller nonenzymatic rate | Saccharomyces cerevisiae |