General Stability | Organism |
---|---|
ammonium sulfate, 1 M, complete stabilization, 6 days at 20°C | Saccharomyces cerevisiae |
cystine, alone: no stabilization, in addition to glutathione: increases stabilizing effect | Saccharomyces cerevisiae |
EDTA stabilizes | Saccharomyces cerevisiae |
ethylene glycol, 10%, stabilizes | Saccharomyces cerevisiae |
glutathione stabilizes, stabilizing effect increased by cystine | Saccharomyces cerevisiae |
glycerol, no stabilization | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell associated | - |
Saccharomyces cerevisiae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cystine + GSH | Saccharomyces cerevisiae | - |
cysteine + GSSG | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
61.6 | - |
beta-hydroxyethyldisulfide | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, pH 5.8, 1 mM glutathione, several months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-hydroxyethyl disulfide + GSH | - |
Saccharomyces cerevisiae | ? | - |
? | |
cystine + GSH | - |
Saccharomyces cerevisiae | cysteine + GSSG | - |
? | |
D-cystine + 2 GSH | - |
Saccharomyces cerevisiae | 2 D-cysteine + GSSG | - |
? | |
diacetyl-L-cystine + GSH | - |
Saccharomyces cerevisiae | ? | - |
? | |
homocystine + GSH | - |
Saccharomyces cerevisiae | ? | - |
? | |
L-cystine + GSH | - |
Saccharomyces cerevisiae | L-cysteine + GSSG | - |
? | |
L-cystine diamide + GSH | - |
Saccharomyces cerevisiae | ? | - |
? | |
L-cystinyldiglycine + GSH | - |
Saccharomyces cerevisiae | L-Cys-Gly-Gly + GSSG | - |
? | |
additional information | at low substrate concentration the reaction rate is greater with L-cystine than with any of the other substances | Saccharomyces cerevisiae | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
pH 7.8 chosen for the assay to achieve smaller nonenzymatic rate | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | pH 6.0: 10% of activity maximum, pH 8.5: activity maximum | Saccharomyces cerevisiae |