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Literature summary for 1.8.4.2 extracted from
- Characterization of Mycobacterium tuberculosis WhiB1/Rv3219 as a protein disulfide reductase (2007), Protein Expr. Purif., 52, 422-432.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain D5alpha | Mycobacterium tuberculosis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14000 | - |
2 * 14000, SDS-PAGE | Mycobacterium tuberculosis |
| 14056 | - |
2 * 14056, unmodified recombinant WhiB1, MALDI-TOF mass spectrometry | Mycobacterium tuberculosis |
| 14284 | - |
2 * 14284, reduced protein after alkylation corresponding to the theoretical mass of the recombinant protein with four iodoacetamide mediated modifications of cysteines, MALDI-TOF mass spectrometry | Mycobacterium tuberculosis |
| 29000 | - |
gel filtration | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-NTA affinity chromatography | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
1.56 U/mg in the cell extract, 8.17 U/mg after Ni2+-NTA chromatography purification, one unit is defined as the amount of protein that produces an increase in 0.1 OD in 1 min at 650 nm | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSH + protein disulfide | - |
Mycobacterium tuberculosis | GSSG + protein-dithiol | - |
? |  |
| GSH + protein disulfide | - |
Mycobacterium tuberculosis Rv3219 | GSSG + protein-dithiol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 14000, SDS-PAGE | Mycobacterium tuberculosis |
| homodimer | 2 * 14056, unmodified recombinant WhiB1, MALDI-TOF mass spectrometry | Mycobacterium tuberculosis |
| homodimer | 2 * 14284, reduced protein after alkylation corresponding to the theoretical mass of the recombinant protein with four iodoacetamide mediated modifications of cysteines, MALDI-TOF mass spectrometry | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| WhiB1 | - |
Mycobacterium tuberculosis |
| WhiB1/Rv3219 | - |
Mycobacterium tuberculosis |
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Release 2023.1 (January 2023)
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