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Literature summary for 1.8.4.2 extracted from

  • Ansorge, S.; Bohley, P.; Kirschke, H.; Langner, J.; Wiederanders, B.; Hanson, H.
    Metabolism of insulin and glucagon. Glutathione-insulin transhydrogenase from microsomes of rat liver (1973), Eur. J. Biochem., 32, 27-35.
    View publication on PubMed
Search for more literature for 1.8.4.2

General Stability

General Stability Organism
EDTA stabilizes during purification Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
 Insulin
-
 Rattus norvegicus
1.43
-
 GSH
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000 55000 gel filtration Rattus norvegicus
60000
-
 SDS-PAGE, a 120000 MW protein also found Rattus norvegicus
120000
-
 SDS-PAGE, MW 60000 determined for the main component Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
458
-
 purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GSH + protein disulfide
-
 Rattus norvegicus GSSG + protein-dithiol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Rattus norvegicus