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Literature summary for 1.8.4.15 extracted from

  • Akiyama, Y.; Kamitani, S.; Kusukawa, N.; Ito, K.
    In vitro catalysis of oxidative folding of disulfide-bonded proteins by the Escherichia coli dsbA (ppfA) gene product (1992), J. Biol. Chem., 267, 22440-22445 .
    View publication on PubMed
Search for more literature for 1.8.4.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain HBlOl or MC4100 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1 mM used in assay conditions Escherichia coli
Zn2+ 0.16 mM used in assay conditions Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000 50000 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose CL-GB column chromatography and Sephacryl S-200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dithiothreitol + reduced bovine ribonuclease A
-
 Escherichia coli ? + oxidized bovine ribonuclease A
-
 ?
GSH + scrambled bovine ribonuclease A
-
 Escherichia coli ?
-
 ?
GSSG + reduced alkaline phosphatase
-
 Escherichia coli 2 GSH + oxidized alkaline phosphatase
-
 ?

Subunits

Subunits Comment Organism
homodimer x * 21000, SDS-PAGE Escherichia coli
homodimer x * 21132, calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
DsbA
-
 Escherichia coli

General Information

General Information Comment Organism
physiological function the enzyme directly catalyzes disulfide bond formation and also has a role in maintaining the bacterial periplasm oxidative Escherichia coli