Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | complete inhibition at 1 mM | Saccharomyces cerevisiae | |
arsenite | strong inhibition | Saccharomyces cerevisiae | |
bovine serum albumin | - |
Saccharomyces cerevisiae | |
DL-methionine sulfoximine | 50% inhibition at 1 mM | Saccharomyces cerevisiae | |
hydroxyethyl disulfide | competitively inhibits the activity with L-(-)-methionine S-oxide about 2.5fold | Saccharomyces cerevisiae | |
iodoacetic acid | complete inhibition at 10 mM | Saccharomyces cerevisiae | |
L-(-)-methionine S-oxide | competitively inhibits the activity with a disulfide about 2.5fold | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
L-(-)-methionine S-oxide | pH 7.0, 22°C | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-(-)-methionine S-oxide + NADPH | Saccharomyces cerevisiae | the enzyme is specific for the L-(-)-stereoisomer | L-methionine + NADP+ + H2O | - |
ir | |
additional information | Saccharomyces cerevisiae | possible pathway, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
two enzyme forms | - |
Purification (Comment) | Organism |
---|---|
two native enzyme forms by ammonium sulfate fractionation, anion exchange chromatography, and a calcium phosphate resin chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-(-)-methionine S-oxide + NADPH | the enzyme is specific for the L-(-)-stereoisomer | Saccharomyces cerevisiae | L-methionine + NADP+ + H2O | - |
ir | |
L-(-)-methionine S-oxide + NADPH | the enzyme is absolutely specific for the L-(-)-stereoisomer | Saccharomyces cerevisiae | L-methionine + NADP+ + H2O | - |
ir | |
additional information | possible pathway, overview | Saccharomyces cerevisiae | ? | - |
? | |
additional information | the purified enzyme may contain thioredoxin | Saccharomyces cerevisiae | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | specific for | Saccharomyces cerevisiae |