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Literature summary for 1.8.4.12 extracted from

  • Sasoni, N.; Hartman, M.; Guerrero, S.; Iglesias, A.; Arias, D.
    Functional characterization of methionine sulfoxide reductases from Leptospira interrogans (2021), Biochim. Biophys. Acta Proteins Proteom., 1869, 140575.
    View publication on PubMed
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) fused to an C-terminal 6Histag Leptospira interrogans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 glutaredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans
0.026
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans
0.21
-
 N-acetyl-L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans
1
-
 L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm dual localization, both in cytoplasm and periplasm Leptospira interrogans 5737
-
periplasm dual localization, both in cytoplasm and periplasm Leptospira interrogans
-
-

Organism

Organism UniProt Comment Textmining
Leptospira interrogans Q72NN2 serovar Copenhageni
-
Leptospira interrogans Fiocruz L1-130 Q72NN2 serovar Copenhageni
-

Purification (Commentary)

Purification (Comment) Organism
-
 Leptospira interrogans

Storage Stability

Storage Stability Organism
-80°C, 8 months, no loss of activity Leptospira interrogans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine-(R)-S-oxide + glutaredoxin glutaredoxin from Homo sapiens Leptospira interrogans L-methionine + glutaredoxin disulfide + H2O
-
 ?
L-methionine-(R)-S-oxide + glutaredoxin glutaredoxin from Homo sapiens Leptospira interrogans Fiocruz L1-130 L-methionine + glutaredoxin disulfide + H2O
-
 ?
L-methionine-(R)-S-oxide + thioredoxin thioredoxin from Leptospira interrogans Leptospira interrogans L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine-(R)-S-oxide + thioredoxin thioredoxin from Leptospira interrogans Leptospira interrogans Fiocruz L1-130 L-methionine + thioredoxin disulfide + H2O
-
 ?
N-acetyl-L-methionine-(R)-S-oxide + glutaredoxin glutaredoxin from Homo sapiens Leptospira interrogans N-acetyl-L-methionine + glutaredoxin disulfide + H2O
-
 ?
N-acetyl-L-methionine-(R)-S-oxide + glutaredoxin glutaredoxin from Homo sapiens Leptospira interrogans Fiocruz L1-130 N-acetyl-L-methionine + glutaredoxin disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
MsrB
-
 Leptospira interrogans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0043
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans
0.0076
-
 L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans
0.014
-
 N-acetyl-L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans
0.0145
-
 glutaredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0076
-
 L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans
0.067
-
 N-acetyl-L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans
0.17
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans
5.2
-
 glutaredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans