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Literature summary for 1.8.4.12 extracted from

  • Han, A.R.; Kim, M.J.; Kwak, G.H.; Son, J.; Hwang, K.Y.; Kim, H.Y.
    Essential role of the linker region in the higher catalytic efficiency of a bifunctional MsrA-MsrB fusion protein (2016), Biochemistry, 55, 5117-5127 .
    View publication on PubMed
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
-
 Treponema denticola

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.3 A resolution, in presence and absence of dithiothreitol Treponema denticola

Protein Variants

Protein Variants Comment Organism
C11S catalytic efficiency is about 140% of wild-type Treponema denticola
D164A catalytic efficiency is about 80% of wild-type Treponema denticola
E165A catalytic efficiency is about 65% to wild-type Treponema denticola
Y163A catalytic efficiency is about 20% of wild-type Treponema denticola
Y163A/E165A catalytic efficiency is about 17% of wild-type Treponema denticola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 dabsyl-L-methionine (R)-S-oxide mutant C11S, pH 7.5, 37°C Treponema denticola
0.04
-
 dabsyl-L-methionine (R)-S-oxide mutant D164A, pH 7.5, 37°C Treponema denticola
0.04
-
 dabsyl-L-methionine (R)-S-oxide mutant E165A, pH 7.5, 37°C Treponema denticola
0.05
-
 dabsyl-L-methionine (R)-S-oxide wild-type, pH 7.5, 37°C Treponema denticola
0.05
-
 dabsyl-L-methionine (R)-S-oxide mutant Y163A, pH 7.5, 37°C Treponema denticola
0.06
-
 dabsyl-L-methionine (R)-S-oxide mutant Y163A//E165A, pH 7.5, 37°C Treponema denticola
0.085
-
 dabsyl-L-methionine (R)-S-oxide isolated MrsB domain, pH 7.5, 37°C Treponema denticola

Organism

Organism UniProt Comment Textmining
Treponema denticola Q73PT7 bifunctional methionine sulfoxide reductase AB, cf. EC 1.8.1.11
-
Treponema denticola ATCC 35405 Q73PT7 bifunctional methionine sulfoxide reductase AB, cf. EC 1.8.1.11
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine (R)-S-oxide + dithiothreitol
-
 Treponema denticola dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
dabsyl-L-methionine (R)-S-oxide + dithiothreitol
-
 Treponema denticola ATCC 35405 dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
51.3
-
 wild-type, melting temperature Treponema denticola
53.6
-
 isolated MrsA domain, melting temperature Treponema denticola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
 dabsyl-L-methionine (R)-S-oxide isolated MrsB domain, pH 7.5, 37°C Treponema denticola
0.025
-
 dabsyl-L-methionine (R)-S-oxide mutant Y163A//E165A, pH 7.5, 37°C Treponema denticola
0.036
-
 dabsyl-L-methionine (R)-S-oxide mutant Y163A, pH 7.5, 37°C Treponema denticola
0.068
-
 dabsyl-L-methionine (R)-S-oxide mutant E165A, pH 7.5, 37°C Treponema denticola
0.083
-
 dabsyl-L-methionine (R)-S-oxide mutant D164A, pH 7.5, 37°C Treponema denticola
0.102
-
 dabsyl-L-methionine (R)-S-oxide mutant C11S, pH 7.5, 37°C Treponema denticola
0.116
-
 dabsyl-L-methionine (R)-S-oxide wild-type, pH 7.5, 37°C Treponema denticola

General Information

General Information Comment Organism
physiological function methionine sulfoxide reductase A (MsrA, EC 1.8.1.11) and B (MsrB, EC 1.8.1.12) are present as a fusion form. The catalytic efficiency of both MsrA and MsrB increases after fusion of the domains and the linker region (iloop) that connects MsrA and MsrB is required for the higher catalytic efficiency of the fusion protein. The iloop mainly interacts with MsrB via hydrogen bonds. The iloop-MsrB interactions are critical to MsrB and MsrA activities Treponema denticola

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.005
-
 dabsyl-L-methionine (R)-S-oxide isolated MrsB domain, pH 7.5, 37°C Treponema denticola
0.42
-
 dabsyl-L-methionine (R)-S-oxide mutant Y163A//E165A, pH 7.5, 37°C Treponema denticola
0.72
-
 dabsyl-L-methionine (R)-S-oxide mutant Y163A, pH 7.5, 37°C Treponema denticola
1.7
-
 dabsyl-L-methionine (R)-S-oxide mutant E165A, pH 7.5, 37°C Treponema denticola
2.08
-
 dabsyl-L-methionine (R)-S-oxide mutant D164A, pH 7.5, 37°C Treponema denticola
2.32
-
 dabsyl-L-methionine (R)-S-oxide wild-type, pH 7.5, 37°C Treponema denticola
3.4
-
 dabsyl-L-methionine (R)-S-oxide mutant C11S, pH 7.5, 37°C Treponema denticola