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Literature summary for 1.8.4.12 extracted from

  • Dai, C.; Liu, L.; Wang, M.H.
    Characterization of a methionine sulfoxide reductase B from tomato (Solanum lycopersicum), and its protecting role in Saccharomyces cerevisiae (2013), Protein J., 32, 39-47.
    View publication on PubMed
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3)cells and in Saccharomyces cerevisiae strain BY4741 Solanum lycopersicum

Protein Variants

Protein Variants Comment Organism
C181S inactive Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0091
-
 dithiothreitol recombinant enzyme, in 50 mM Tris-HCl, pH 8.0, at 37°C Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Solanum lycopersicum 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21200
-
 x * 21200, calculated from amino acid sequence Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-affinity column chromatography Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
flower high expression Solanum lycopersicum
-
fruit high expression in red mature fruits Solanum lycopersicum
-
leaf high expression Solanum lycopersicum
-
root low expression Solanum lycopersicum
-
stem low expression Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine (R)-sulfoxide + dithiothreitol
-
 Solanum lycopersicum dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
L-methionine (R)-sulfoxide + dithiothreitol
-
 Solanum lycopersicum L-methionine + dithiothreitol disulfide + H2O
-
 ?
additional information isoform MSRB1 exhibits no activity in thioredoxin dependent system Solanum lycopersicum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 21200, calculated from amino acid sequence Solanum lycopersicum

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase B
-
 Solanum lycopersicum
MsrB
-
 Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.09
-
 dithiothreitol recombinant enzyme, in 50 mM Tris-HCl, pH 8.0, at 37°C Solanum lycopersicum

pI Value

Organism Comment pI Value Maximum pI Value
Solanum lycopersicum calculated from amino acid sequence
-
 8.9

General Information

General Information Comment Organism
physiological function the MSRB1 gene plays a critical role in protecting against oxidative stress Solanum lycopersicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10
-
 dithiothreitol recombinant enzyme, in 50 mM Tris-HCl, pH 8.0, at 37°C Solanum lycopersicum