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Literature summary for 1.8.4.12 extracted from

  • Grimaud, R.; Ezraty, B.; Mitchell, J.K.; Lafitte, D.; Briand, C.; Derrick, P.J.; Barras, F.
    Repair of oxidized proteins: identification of a new methionine sulfoxide reductase (2001), J. Biol. Chem., 276, 48915-48920.
    View publication on PubMed
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
gene msrB, overexpression of wild-type enzyme and mutant enzymes in Escherichia coli strain DH5alpha Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine (R)-sulfoxide + thioredoxin Escherichia coli enzyme is involved in repairing of oxidized methionine residues in proteins L-methionine + thioredoxin disulfide + H2O
-
 ?
additional information Escherichia coli enzyme contributes to resistance against cadmium, physiological role ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene msrB, enzyme SelR which is a selenoprotein, enzyme is organized in a fusion protein together with MsrA, EC 1.8.4.B2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain DH5alpha Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine (R)-sulfoxide + thioredoxin enzyme is involved in repairing of oxidized methionine residues in proteins Escherichia coli L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine (R)-sulfoxide + thioredoxin MsrB is specific for the R-form of the substrate Escherichia coli L-methionine + thioredoxin disulfide + H2O
-
 ?
additional information enzyme contributes to resistance against cadmium, physiological role Escherichia coli ?
-
 ?
oxidized calmodulin + thioredoxin enzyme reduces L-methionine (R)-sulfoxide of the protein substrate Escherichia coli partially reduced calmodulin + thioredoxin disulfide
-
 ?

Subunits

Subunits Comment Organism
additional information MsrB has a highly conserved sequence among organisms Escherichia coli

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase
-
 Escherichia coli
MsrB
-
 Escherichia coli
SelR
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 L-methionine (R)-sulfoxide in vitro, substrate free L-methionine (R)-sulfoxide Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
 Escherichia coli