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Literature summary for 1.8.4.12 extracted from

  • Koc, A.; Gasch, A.P.; Rutherford, J.C.; Kim, H.Y.; Gladyshev, V.N.
    Methionine sulfoxide reductase regulation of yeast lifespan reveals reactive oxygen species-dependent and -independent components of aging (2004), Proc. Natl. Acad. Sci. USA, 101, 7999-8004.
    View publication on PubMed
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of MsrB in a yeast strain, expression of MsrB as N-terminally 6His-tagged protein in Escherichia coli strain BL-21 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine (R)-sulfoxide + thioredoxin Saccharomyces cerevisiae MsrB specifically reduces the R-form of methionine sulfoxide L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine (R)-sulfoxide + thioredoxin Saccharomyces cerevisiae BY4741 MsrB specifically reduces the R-form of methionine sulfoxide L-methionine + thioredoxin disulfide + H2O
-
 ?
additional information Saccharomyces cerevisiae the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview ?
-
 ?
additional information Saccharomyces cerevisiae BY4741 the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 enzyme MsrB
-
Saccharomyces cerevisiae BY4741
-
 enzyme MsrB
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 recombinant activity in overexpressing yeast cells Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine (R)-sulfoxide + thioredoxin MsrB specifically reduces the R-form of methionine sulfoxide Saccharomyces cerevisiae dabsyl-L-methionine + thioredoxin disulfide + H2O
-
 ?
dabsyl-L-methionine (R)-sulfoxide + thioredoxin MsrB specifically reduces the R-form of methionine sulfoxide Saccharomyces cerevisiae BY4741 dabsyl-L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine (R)-sulfoxide + thioredoxin MsrB specifically reduces the R-form of methionine sulfoxide Saccharomyces cerevisiae L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine (R)-sulfoxide + thioredoxin MsrB specifically reduces the R-form of methionine sulfoxide Saccharomyces cerevisiae BY4741 L-methionine + thioredoxin disulfide + H2O
-
 ?
additional information the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview Saccharomyces cerevisiae ?
-
 ?
additional information the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview Saccharomyces cerevisiae BY4741 ?
-
 ?

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase
-
 Saccharomyces cerevisiae
MsrB
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
 Saccharomyces cerevisiae