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Literature summary for 1.8.4.12 extracted from
- The mirrored methionine sulfoxide reductases of Neisseria gonorrhoeae pilB (2002), Nat. Struct. Biol., 9, 348-352.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| CBS-1, overexpression of the His-tagged enzyme in Escherichia coli | Homo sapiens |
| overexpression of the full length tandem enzyme, the MsrA, and the MsrB domains, all His-tagged, in Escherichia coli | Neisseria gonorrhoeae |
| yeaA, overexpression of the His-tagged enzyme in Escherichia coli | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant detagged MsrB domain containing SeMet39, hanging drop vapour-diffusion method, 15 mg/ml protein in 20 mM Tris, pH 8.5, 10% v/v glycerol, against a well solution containing 0.1 M sodium cacodylate, pH 6.5, 30% w/v PEG 4000, room temperature, X-ray diffraction structure determination and analysis at 1.8 A resolution, modeling | Neisseria gonorrhoeae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0A746 | - |
- |
| Homo sapiens | Q9Y3D2 | - |
- |
| Neisseria gonorrhoeae | P14930 | enzyme contains MsrA and MsrB domains; PilB enzyme has 2 catalytic domains showing MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity, respectively, the MsrB domain contains a selenomethionine at position 39 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli | Escherichia coli |
| recombinant His-tagged enzyme from Escherichia coli | Homo sapiens |
| recombinant His-tagged full length tandem enzyme, MsrA, and MsrB domains from Escherichia coli, tags are removed by thrombin digestion | Neisseria gonorrhoeae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | reaction mechanism, modeling of substrate binding at the active site, Cys444 and Cys495 are involved | Neisseria gonorrhoeae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.075 | - |
purified recombinant MsrA/MsrB tandem domain, substrate L-methionine (R)-sulfoxide | Neisseria gonorrhoeae |
| 0.085 | - |
purified recombinant CBS-1, substrate L-methionine (R)-sulfoxide | Escherichia coli |
| 0.092 | - |
purified recombinant CBS-1, substrate L-methionine (R)-sulfoxide | Homo sapiens |
| 0.11 | - |
purified recombinant MsrB domain alone, substrate L-methionine (R)-sulfoxide | Neisseria gonorrhoeae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-methionine (R)-sulfoxide + thioredoxin | MsrB activity of the tandem domains of PilB, the MsrB domain alone does not utilize the S-isomer | Neisseria gonorrhoeae | L-methionine + thioredoxin disulfide + H2O | - |
? |  |
| L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-isomer, no activity with the S-isomer | Homo sapiens | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-isomer, no activity with the S-isomer | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| additional information | the tandem domains of PilB also possess MsrA activity utilizing L-methionine (S)-sulfoxide as substrate, the MsrA domain alone does very poorly utilize the R-isomer | Neisseria gonorrhoeae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| additional information | enzyme domain and active site structure, MsrB domain comprises residues 375-522, overview | Neisseria gonorrhoeae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CBS-1 | - |
Homo sapiens |
| methionine sulfoxide reductase | - |
Neisseria gonorrhoeae |
| methionine sulfoxide reductase | - |
Homo sapiens |
| methionine sulfoxide reductase | - |
Escherichia coli |
| methionine sulfoxide reductase B | - |
Neisseria gonorrhoeae |
| MsrB | - |
Neisseria gonorrhoeae |
| PilB | - |
Neisseria gonorrhoeae |
| YeaA | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Neisseria gonorrhoeae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
assay at | Neisseria gonorrhoeae |
| 6.9 | - |
assay at | Homo sapiens |
| 6.9 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | - |
Neisseria gonorrhoeae | |
| thioredoxin | - |
Homo sapiens | |
| thioredoxin | - |
Escherichia coli | |
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