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Literature summary for 1.8.4.12 extracted from

  • Spector, D.; Etienne, F.; Brot, N.; Weissbach, H.
    New membrane-associated and soluble peptide methionine sulfoxide reductases in Escherichia coli (2003), Biochem. Biophys. Res. Commun., 302, 284-289.
    View publication on PubMed
Search for more literature for 1.8.4.12

Protein Variants

Protein Variants Comment Organism
additional information construction of a MsrA/MsrB double mutant Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein-L-methionine-(R)-sulfoxide + thioredoxin Escherichia coli MsrB is specific for the R-form, the membrane-associated isozyme reduces both R- and S-stereoisomers of methionine sulfoxide, N-acetylmethionine sulfoxide, and D-Ala-Met-enkephalin protein-L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 wild-type strain MC1061, isozyme MsrB and a membrane-associated isozyme
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00022
-
 MsrB, substrate N-acetyl-L-methionine-(R)-sulfoxide Escherichia coli
0.0004
-
 membrane vesicles, substrate N-acetyl-L-methionine-(R)-sulfoxide Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine (R)-sulfoxide + thioredoxin MsrB is specific for the R-form, the membrane-associated isozyme reduces both R- and S-stereoisomers of methionine sulfoxide in proteins Escherichia coli L-methionine + thioredoxin disulfide + H2O
-
 ?
additional information the enzymes utilize free and protein-bound L-methionine and N-acetyl-L-methionine as substrates, the membrane-associated isozyme also shows MsrA activity utilizing L-methionine (S)-sulfoxide and N-acetyl-L-methionine (S)-sulfoxide as substrates Escherichia coli ?
-
 ?
N-acetyl-L-methionine (R)-sulfoxide + thioredoxin MsrB is specific for the R-form, the membrane-associated isozyme reduces both R- and S-stereoisomers Escherichia coli N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
 ?
protein-L-methionine-(R)-sulfoxide + thioredoxin MsrB is specific for the R-form, the membrane-associated isozyme reduces both R- and S-stereoisomers of methionine sulfoxide, N-acetylmethionine sulfoxide, and D-Ala-Met-enkephalin Escherichia coli protein-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
MsrB
-
 Escherichia coli
peptide methionine sulfoxide reductase
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
dithiothreitol MsrB can also utilize DTT as reductant, the membrane-isozyme shows only poor activity Escherichia coli
thioredoxin
-
 Escherichia coli