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Literature summary for 1.8.4.12 extracted from

  • Bar-Noy, S.; Moskovitz, J.
    Mouse methionine sulfoxide reductase B: effect of selenocysteine incorporation on its activity and expression of the seleno-containing enzyme in bacterial and mammalian cells (2002), Biochem. Biophys. Res. Commun., 297, 956-961.
    View publication on PubMed
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild-type and mutants in Escherichia coli, expression as N- or C-terminally 6His-tagged protein lowers the recombinant expression level to 3% of total enzyme expressed, labeling of expressed wild-type with 75SeMet Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information construction of a non-selenomethionine mutant of MsrB by site-directed mutagenesis, exchange of the selenomethionine by Cys, Ala, or Ser, the Cys-enzyme shows reduced activity, the Ser- and Ala-enzymes are inactive, substrate specificity, overview Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13000
-
 x * 13000, native wild-type MsrB, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine (R)-sulfoxide + thioredoxin Mus musculus together with the enzyme MsrA, EC 1.8.4.11, which is absolutely specific for the S-form substrate, the enzyme can repair methionine-damaged proteins and salvage free methionine under oxidative stress int the living cell L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 selenomethionine-containing enzyme MsrB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged wild-type and mutant MsrB to homogeneity Mus musculus

Reaction

Reaction Comment Organism Reaction ID
L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O selenomethionine is essential for MsrB activity Mus musculus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine (R)-sulfoxide + thioredoxin together with the enzyme MsrA, EC 1.8.4.11, which is absolutely specific for the S-form substrate, the enzyme can repair methionine-damaged proteins and salvage free methionine under oxidative stress int the living cell Mus musculus L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine (R)-sulfoxide + thioredoxin the native MsrB as well as the recombinant modified MsrB show absolute specificity for the R-form of free and protein-bound methionine sulfoxide, no activity with the S-form Mus musculus L-methionine + thioredoxin disulfide + H2O
-
 ?
additional information substrate specificity Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 13000, native wild-type MsrB, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase B
-
 Mus musculus
MsrB
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
 Mus musculus