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Literature summary for 1.8.4.12 extracted from
- Purification and characterization of methionine sulfoxide reductases from mouse and Staphylococcus aureus and their substrate stereospecificity (2002), Biochem. Biophys. Res. Commun., 290, 62-65.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene msrB with the codon for selenomehinonine is exchanged for methionine, overexpression in Escherichia coli | Mus musculus |
| pilB is transcribed as polycistronic transcript, overexpression of PilB as His-tagged protein in Escherichia coliBL21(DE3) | Staphylococcus aureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
MsrB is a selenoprotein | - |
| Staphylococcus aureus | - |
PilB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification of native MsrB from liver, recombinant modified MsrB from Escherichia coli | Mus musculus |
| recombinant His-tagged PilB from Escherichia coli by nickel affinity chromatography | Staphylococcus aureus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Mus musculus | - |
| cerebellum | - |
Mus musculus | - |
| kidney | - |
Mus musculus | - |
| liver | highest activity | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
tissue specific activity, activity in tissues of MsrA-deficient mutant mice | Mus musculus |
| 0.0007 | - |
kidney, substrate L-methionine (R)-sulfoxide | Mus musculus |
| 0.0019 | - |
liver, substrate L-methionine (R)-sulfoxide | Mus musculus |
| 1.78 | - |
purified recombinant PilB, substrate L-methionine (R)-sulfoxide | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dabsyl-L-methionine (R)-sulfoxide + thioredoxin | the native MsrB as well as the recombinant modified MsrB show absolute specificity for the R-form of free and protein-bound methionine sulfoxide, no activity with the S-form | Mus musculus | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? |  |
| L-methionine (R)-sulfoxide + thioredoxin | the native MsrB as well as the recombinant modified MsrB show absolute specificity for the R-form of free and protein-bound methionine sulfoxide, no activity with the S-form | Mus musculus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | PilB shows absolute specificity for the R-form of free and protein-bound methionine sulfoxide | Staphylococcus aureus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methionine sulfoxide reductase | - |
Mus musculus |
| methionine sulfoxide reductase | - |
Staphylococcus aureus |
| MsrB | - |
Mus musculus |
| MsrB | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
| 37 | - |
assay at | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mus musculus |
| 7.4 | - |
assay at | Staphylococcus aureus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | - |
Mus musculus | |
| thioredoxin | - |
Staphylococcus aureus | |
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