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Literature summary for 1.8.4.11 extracted from

  • Zhu, Q.; El-Mergawy, R.G.; Zhou, Y.; Chen, C.; Heinemann, S.H.; Schoenherr, R.; Robaa, D.; Sippl, W.; Scriba, G.K.
    Stereospecific capillary electrophoresis assays using pentapeptide substrates for the study of Aspergillus nidulans methionine sulfoxide reductase A and mutant enzymes (2016), Electrophoresis, 37, 2083-2090 .
    View publication on PubMed
Search for more literature for 1.8.4.11

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling. The conserved residue Glu99 is buried in the Met-S-(O) groove, which might contribute to the correct placing of substrates. Residue Asp134 does not form hydrogen bonds with the substrates but only within the enzyme Aspergillus nidulans

Protein Variants

Protein Variants Comment Organism
D134E most active among the mutant enzymes tested, less active than wild-type Aspergillus nidulans
D134N lowest activity among all mutants tested for ac-L-Lys-L-Asn-l-Met(O)-L-Asp-L-Lys-dinitrophenol Aspergillus nidulans
E99Q lowest activity towards all substrates tested except for ac-L-Lys-L-Asn-l-Met(O)-L-Asp-L-Lys-dinitrophenol Aspergillus nidulans

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
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Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ac-L-Lys-L-Asn-L-Met(O)-L-Asp-L-Lys-dinitrophenol + dithiothreitol
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 Aspergillus nidulans ac-L-Lys-L-Asn-L-Met-L-Asp-L-Lys-dinitrophenol + dithiothreitol disulfide + H2O
-
 ?
ac-L-Lys-L-Asp-L-Met(O)-L-Asn-L-Lys-dinitrophenol + dithiothreitol
-
 Aspergillus nidulans ac-L-Lys-L-Asp-L-Met-L-Asn-L-Lys-dinitrophenol + dithiothreitol disulfide + H2O
-
 ?
ac-L-Lys-L-Asp-L-Met(O)-L-Asp-L-Lys-dinitrophenol + dithiothreitol
-
 Aspergillus nidulans ac-L-Lys-L-Asp-L-Met-L-Asp-L-Lys-dinitrophenol + dithiothreitol disulfide + H2O
-
 ?
ac-L-Lys-L-Phe-L-Met(O)-L-Lys-L-Lys-dinitrophenol + dithiothreitol
-
 Aspergillus nidulans ac-L-Lys-L-Phe-L-Met-L-Lys-L-Lys-dinitrophenol + dithiothreitol disulfide + H2O
-
 ?