Cloned (Comment) | Organism |
---|---|
- |
Treponema denticola |
Crystallization (Comment) | Organism |
---|---|
to 2.3 A resolution, in presence and absence of dithiothreitol | Treponema denticola |
Protein Variants | Comment | Organism |
---|---|---|
C285S | kcat value is similar to that of wild-type, while its Km value is slightly higher than that of the wild type | Treponema denticola |
D164A | catalytic efficiency is about 120% of wild-type | Treponema denticola |
E165A | catalytic efficiency is similar to wild-type | Treponema denticola |
Y163A | catalytic efficiency is about 60% of wild-type | Treponema denticola |
Y163A/E165A | catalytic efficiency is about 8% of wild-type | Treponema denticola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
dabsyl-L-methionine (S)-S-oxide | mutant D164A, pH 7.5, 37°C | Treponema denticola | |
0.15 | - |
dabsyl-L-methionine (S)-S-oxide | mutant E165A, pH 7.5, 37°C | Treponema denticola | |
0.17 | - |
dabsyl-L-methionine (S)-S-oxide | mutant Y163A, pH 7.5, 37°C | Treponema denticola | |
0.18 | - |
dabsyl-L-methionine (S)-S-oxide | wild-type, pH 7.5, 37°C | Treponema denticola | |
0.3 | - |
dabsyl-L-methionine (S)-S-oxide | isolated MrsA domain, pH 7.5, 37°C | Treponema denticola | |
0.3 | - |
dabsyl-L-methionine (S)-S-oxide | mutant C285S, pH 7.5, 37°C | Treponema denticola | |
1.03 | - |
dabsyl-L-methionine (S)-S-oxide | mutant Y163A//E165A, pH 7.5, 37°C | Treponema denticola |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Treponema denticola | Q73PT7 | bifunctional methionine sulfoxide reductase AB, cf. EC 1.8.1.12 | - |
Treponema denticola ATCC 35405 | Q73PT7 | bifunctional methionine sulfoxide reductase AB, cf. EC 1.8.1.12 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dabsyl-L-methionine (S)-S-oxide + dithiothreitol | - |
Treponema denticola | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
dabsyl-L-methionine (S)-S-oxide + dithiothreitol | - |
Treponema denticola ATCC 35405 | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
51.3 | - |
wild-type, melting temperature | Treponema denticola |
53.6 | - |
isolated MrsA domain, melting temperature | Treponema denticola |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
dabsyl-L-methionine (S)-S-oxide | isolated MrsA domain, pH 7.5, 37°C | Treponema denticola | |
0.045 | - |
dabsyl-L-methionine (S)-S-oxide | mutant Y163A//E165A, pH 7.5, 37°C | Treponema denticola | |
0.068 | - |
dabsyl-L-methionine (S)-S-oxide | mutant Y163A, pH 7.5, 37°C | Treponema denticola | |
0.096 | - |
dabsyl-L-methionine (S)-S-oxide | mutant E165A, pH 7.5, 37°C | Treponema denticola | |
0.108 | - |
dabsyl-L-methionine (S)-S-oxide | mutant C285S, pH 7.5, 37°C | Treponema denticola | |
0.11 | - |
dabsyl-L-methionine (S)-S-oxide | mutant D164A, pH 7.5, 37°C | Treponema denticola | |
0.12 | - |
dabsyl-L-methionine (S)-S-oxide | wild-type, pH 7.5, 37°C | Treponema denticola |
General Information | Comment | Organism |
---|---|---|
physiological function | methionine sulfoxide reductase A (MsrA, EC 1.8.1.11) and B (MsrB, EC 1.8.1.12) are present as a fusion form. The catalytic efficiency of both MsrA and MsrB increases after fusion of the domains and the linker region (iloop) that connects MsrA and MsrB is required for the higher catalytic efficiency of the fusion protein. The iloop mainly interacts with MsrB via hydrogen bonds. The iloop-MsrB interactions are critical to MsrB and MsrA activities | Treponema denticola |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
dabsyl-L-methionine (S)-S-oxide | mutant Y163A//E165A, pH 7.5, 37°C | Treponema denticola | |
0.4 | - |
dabsyl-L-methionine (S)-S-oxide | mutant Y163A, pH 7.5, 37°C | Treponema denticola | |
0.64 | - |
dabsyl-L-methionine (S)-S-oxide | mutant E165A, pH 7.5, 37°C | Treponema denticola | |
0.667 | - |
dabsyl-L-methionine (S)-S-oxide | wild-type, pH 7.5, 37°C | Treponema denticola | |
0.786 | - |
dabsyl-L-methionine (S)-S-oxide | mutant D164A, pH 7.5, 37°C | Treponema denticola | |
87 | - |
dabsyl-L-methionine (S)-S-oxide | isolated MrsA domain, pH 0.75, 37°C | Treponema denticola | |
360 | - |
dabsyl-L-methionine (S)-S-oxide | mutant C285S, pH 7.5, 37°C | Treponema denticola |