Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
expression in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
M229L | mutant is insensitive to hyperoxidation by H2O2 | Mus musculus |
M229V | mutation to corresponding residue of human enzyme, which is not hyperoxidized by H2O2. Mutant is insensitive to hyperoxidation | Mus musculus |
V231M | mutation to corresponding residue of mouse enzyme, which is hyperoxidized by H2O2. Mutant is sensitive to hyperoxidation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | in the mouse enzyme, the sulfenic acid formed at the catalytic Cys residue is vulnerable to irreversible oxidation to cysteine sulfinic acid resulting in inactivation. Hyperoxidation is controlled by the presence or absence of residue Met229 in the carboxyl terminal domain. Mouse msrA becomes insensitive to hyperoxidation when the Met229 is mutated. Hyperoxidation occurs so long as the methionine is located within the 14 carboxyl terminal residues. Met229 may form a stable, non-covalent bond with Trp74 at the active site, preventing formation of a protective sulfenylamide with Cys72 sulfenic acid | Mus musculus | |
additional information | contrary to the mouse enzmye, human MsrA is not sensitive to hyperoxidation by H2O2 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
mitochondrion | - |
Mus musculus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UJ68 | - |
- |
Mus musculus | Q9D6Y7 | - |
- |