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Literature summary for 1.8.4.11 extracted from
- The minimal gene set member msrA, encoding peptide methionine sulfoxide reductase, is a virulence determinant of the plant pathogen Erwinia chrysanthemi (1999), Proc. Natl. Acad. Sci. USA, 96, 887-892.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene msrA, DNA and amino acid sequence determination and analysis, expression of His6-tagged MsrA in Escherichia coli strain BL21 | Dickeya chrysanthemi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Dickeya chrysanthemi | MsrA is a virulence determinant for the plant pathogen required for full virulence | ? | - |
? |  |
| peptide-L-methionine-(S)-S-oxide + thioredoxin | Dickeya chrysanthemi | stereospecific reduction of protein-bound methionine (S)-sulfoxide residues, the enzyme is involved in oxidized protein repair | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dickeya chrysanthemi | - |
gene msrA, wild-type strain 3937 or RH7006 or pin14 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged MsrA from Escherichia coli strain BL21 by nickel affinity chromatography to homogeneity | Dickeya chrysanthemi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
virulence on chicory leaves of wild-type and recombinant strains, overview | Dickeya chrysanthemi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | MsrA is a virulence determinant for the plant pathogen required for full virulence | Dickeya chrysanthemi | ? | - |
? | |
| peptide-L-methionine-(S)-S-oxide + thioredoxin | stereospecific reduction of protein-bound methionine (S)-sulfoxide residues, the enzyme is involved in oxidized protein repair | Dickeya chrysanthemi | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| peptide-L-methionine-(S)-S-oxide + thioredoxin | stereospecific reduction of protein-bound methionine (S)-sulfoxide residues | Dickeya chrysanthemi | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MsrA | - |
Dickeya chrysanthemi |
| peptide methionine sulfoxide reductase | - |
Dickeya chrysanthemi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Dickeya chrysanthemi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Dickeya chrysanthemi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | - |
Dickeya chrysanthemi | |
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