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Literature summary for 1.8.4.11 extracted from

  • Moskovitz, J.; Flescher, E.; Berlett, B.S.; Azare, J.; Poston, J.M.; Stadtman, E.R.
    Overexpression of peptide-methionine sulfoxide reductase in Saccharomyces cerevisiae and human T cells provides them with high resistance to oxidative stress (1998), Proc. Natl. Acad. Sci. USA, 95, 14071-14075.
    View publication on PubMed
Search for more literature for 1.8.4.11

Cloned(Commentary)

Cloned (Comment) Organism
gene msrA, subcloning in Escherichia coli, stable functional overexpression of MsrA in Saccharomyces cerevisiae and human T cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information overexpression of peptide-methionine sulfoxide reductase in Saccharomyces cerevisiae, resulting in a strain with 25fold increased activity, and Molt-4 human lymphocyte cells provides them with high resistance to oxidative stress by H2O2, paraquat, or 2,2'-azobis-(2-amidinopropane) dihydrochloride, the recombinant yeast strain grows better and and shows a higher survival rate compared to the wild-type parent strain Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae MsrA protects the cell against damage caused by oxidative stress through treatment with H2O2, paraquat, or 2,2'-azobis-(2-amidinopropane) dihydrochloride ?
-
 ?
peptide-L-methionine-(S)-S-oxide + thioredoxin Saccharomyces cerevisiae
-
 peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 gene msrA
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 cell survival rates after treatement with H2O2, paraquat, or 2,2'-azobis-(2-amidinopropane) dihydrochloride Saccharomyces cerevisiae
0.000015
-
 wild-type Saccharomyces cerevisiae strain, substrate L-methionine-(S)-S-oxide Saccharomyces cerevisiae
0.000068
-
 wild-type Saccharomyces cerevisiae strain, substrate dabsyl-L-methionine-(S)-S-oxide Saccharomyces cerevisiae
0.0003
-
 recombinant Saccharomyces cerevisiae strain overexpressing MsrA, substrate L-methionine-(S)-S-oxide Saccharomyces cerevisiae
0.0017
-
 recombinant Saccharomyces cerevisiae strain overexpressing MsrA, substrate dabsyl-L-methionine-(S)-S-oxide Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine-(S)-S-oxide + thioredoxin
-
 Saccharomyces cerevisiae dabsyl-L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine-(S)-S-oxide + thioredoxin
-
 Saccharomyces cerevisiae L-methionine + thioredoxin disulfide + H2O
-
 ?
additional information MsrA protects the cell against damage caused by oxidative stress through treatment with H2O2, paraquat, or 2,2'-azobis-(2-amidinopropane) dihydrochloride Saccharomyces cerevisiae ?
-
 ?
peptide-L-methionine-(S)-S-oxide + thioredoxin
-
 Saccharomyces cerevisiae peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
MsrA
-
 Saccharomyces cerevisiae
peptide-methionine sulfoxide reductase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
 Saccharomyces cerevisiae