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Literature summary for 1.8.4.11 extracted from
- Crystallization and preliminary X-ray diffraction studies of the peptide methionine sulfoxide reductase from Escherichia coli (2000), Acta Crystallogr. Sect. D, 56, 1194-1197.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene msrA, expression in strain B834(DE3) | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| 15-50 mg/ml purified recombinant MsrA in 50 mM Tris-HCl, pH 8.0, 2 mM EDTA, and 10 mM DTT, hanging drop vapour diffusion method, droplet size is 0.004-0.008 ml, equal volumes of protein and precipitant solution, X-ray diffraction structure determination and analysis at 1.9 A resolution | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| peptide-L-methionine-(S)-S-oxide + thioredoxin | Escherichia coli | MsrA is involved in the antioxidant defense | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant MsrA from strain B834(DE3) | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| peptide-L-methionine-(S)-S-oxide + DTT | - |
Escherichia coli | peptide-L-methionine + DTT disulfide + H2O | - |
? | |
| peptide-L-methionine-(S)-S-oxide + thioredoxin | MsrA is involved in the antioxidant defense | Escherichia coli | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| peptide-L-methionine-(S)-S-oxide + thioredoxin | - |
Escherichia coli | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MsrA | - |
Escherichia coli |
| peptide methionine sulfoxide reductase | - |
Escherichia coli |
| PMSR | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Escherichia coli | |
| thioredoxin | - |
Escherichia coli | |
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