Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.4.11 extracted from

  • Weissbach, H.; Etienne, F.; Hoshi, T.; Heinemann, S.H.; Lowther, W.T.; Matthews, B.; St John, G.; Nathan, C.; Brot, N.
    Peptide methionine sulfoxide reductase: structure, mechanism of action, and biological function (2002), Arch. Biochem. Biophys., 397, 172-178.
    View publication on PubMed
Search for more literature for 1.8.4.11

Cloned(Commentary)

Cloned (Comment) Organism
gene msrA Escherichia coli

Protein Variants

Protein Variants Comment Organism
C52S site-directed mutagenesis, reduced activity compared to the wild-type, no complementation of a msrA knockout mutant Escherichia coli
additional information a knockout mutant shows reduced ability to attach to host lung and vein epithelial cells Streptococcus pneumoniae
additional information construction of knockout mutants which show higher sensitivity to hydrogen peroxide compared to wild-type cells which can be compensated by complementation with the wild-type msrA gene from either Escherichia coli or Mycobacterium tuberculosis, but a mutant C52S msrA gene cannot restore activity in the knockout mutant strain, mutants show reduced type 1 fimbriae-mediated mannose-dependent agglutination of erythrocytes Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
calmodulin-L-methionine (S)-sulfoxide + thioredoxin Escherichia coli
-
 calmodulin-L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine (S)-sulfoxide + thioredoxin Streptococcus pneumoniae
-
 L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine (S)-sulfoxide + thioredoxin Escherichia coli substrates are several peptides and proteins, overview L-methionine + thioredoxin disulfide + H2O
-
 ?
additional information Escherichia coli the enzyme is important in protection of the cell against oxidative damage by oxidation of methionine residues in proteins, biological function ?
-
 ?
additional information Streptococcus pneumoniae the enzyme is important in protection of the cell against oxidative damage by oxidation of methionine residues in proteins, biological function ?
-
 ?
ribosomal protein L12-L-methionine (S)-sulfoxide + thioredoxin Escherichia coli
-
 ribosomal protein L12-L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Streptococcus pneumoniae
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O reaction mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
calmodulin-L-methionine (S)-sulfoxide + thioredoxin
-
 Escherichia coli calmodulin-L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine (S)-sulfoxide + 2 dithiothreitol
-
 Escherichia coli L-methionine + dithiothreitol disulfide + H2O
-
 ?
L-methionine (S)-sulfoxide + thioredoxin
-
 Escherichia coli L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine (S)-sulfoxide + thioredoxin
-
 Streptococcus pneumoniae L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine (S)-sulfoxide + thioredoxin substrates are several peptides and proteins, overview Escherichia coli L-methionine + thioredoxin disulfide + H2O
-
 ?
additional information MsrA is specific for the S-form of the substrate Escherichia coli ?
-
 ?
additional information the enzyme is important in protection of the cell against oxidative damage by oxidation of methionine residues in proteins, biological function Escherichia coli ?
-
 ?
additional information the enzyme is important in protection of the cell against oxidative damage by oxidation of methionine residues in proteins, biological function Streptococcus pneumoniae ?
-
 ?
ribosomal protein L12-L-methionine (S)-sulfoxide + thioredoxin
-
 Escherichia coli ribosomal protein L12-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
MsrA
-
 Escherichia coli
MsrA
-
 Streptococcus pneumoniae
peptide methionine sulfoxide reductase
-
 Escherichia coli
peptide methionine sulfoxide reductase
-
 Streptococcus pneumoniae

Cofactor

Cofactor Comment Organism Structure
dithiothreitol utilized in vitro Escherichia coli
thioredoxin physiologic cofactor Escherichia coli