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Literature summary for 1.8.4.11 extracted from

  • Hansel, A.; Kuschel, L.; Hehl, S.; Lemke, C.; Agricola, H.J.; Hoshi, T.; Heinemann, S.H.
    Mitochondrial targeting of the human peptide methionine sulfoxide reductase (MSRA), an enzyme involved in the repair of oxidized proteins (2002), FASEB J., 16, 911-913.
    View publication on PubMed
Search for more literature for 1.8.4.11

Cloned(Commentary)

Cloned (Comment) Organism
expression of GFP-tagged wild-type MsrA and several GFP-tagged deletion mutants in several mammalian cell lines, overview, expression of His-tagged wild-type and DELTA(1-22) deletion mutant in Escherichia coli strain M15 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion MsrA contains a mitochondrial targeting sequence which is not necessary for catalytic activity Homo sapiens 5739
-
additional information subcellular localization analysis, the subcellular localization is not tissue-dependent Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptide-L-methionine-(S)-S-oxide + thioredoxin Homo sapiens stereospecific reduction, the enzyme is involved in repair of oxidized proteins peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UJ68
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and DELTA(1-22) deletion mutant from Escherichia coli strain M15 by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptide-L-methionine-(S)-S-oxide + DTT stereospecific reduction Homo sapiens peptide-L-methionine + DTT disulfide + H2O
-
 ?
peptide-L-methionine-(S)-S-oxide + thioredoxin stereospecific reduction, the enzyme is involved in repair of oxidized proteins Homo sapiens peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?
peptide-L-methionine-(S)-S-oxide + thioredoxin stereospecific reduction Homo sapiens peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
MsrA
-
 Homo sapiens
peptide methionine sulfoxide reductase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
dithiothreitol
-
 Homo sapiens
thioredoxin
-
 Homo sapiens