Literature summary for 1.8.4.11 extracted from

Hansel, A.; Kuschel, L.; Hehl, S.; Lemke, C.; Agricola, H.J.; Hoshi, T.; Heinemann, S.H.
Mitochondrial targeting of the human peptide methionine sulfoxide reductase (MSRA), an enzyme involved in the repair of oxidized proteins (2002), FASEB J., 16, 911-913.

Search for more literature for 1.8.4.11

Cloned(Commentary)

Cloned (Comment)	Organism
expression of GFP-tagged wild-type MsrA and several GFP-tagged deletion mutants in several mammalian cell lines, overview, expression of His-tagged wild-type and DELTA(1-22) deletion mutant in Escherichia coli strain M15	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	MsrA contains a mitochondrial targeting sequence which is not necessary for catalytic activity	Homo sapiens	5739	-
additional information	subcellular localization analysis, the subcellular localization is not tissue-dependent	Homo sapiens	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
peptide-L-methionine-(S)-S-oxide + thioredoxin	Homo sapiens	stereospecific reduction, the enzyme is involved in repair of oxidized proteins	peptide-L-methionine + thioredoxin disulfide + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9UJ68	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged wild-type and DELTA(1-22) deletion mutant from Escherichia coli strain M15 by nickel affinity chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
peptide-L-methionine-(S)-S-oxide + DTT	stereospecific reduction	Homo sapiens	peptide-L-methionine + DTT disulfide + H2O	-	?
peptide-L-methionine-(S)-S-oxide + thioredoxin	stereospecific reduction	Homo sapiens	peptide-L-methionine + thioredoxin disulfide + H2O	-	?
peptide-L-methionine-(S)-S-oxide + thioredoxin	stereospecific reduction, the enzyme is involved in repair of oxidized proteins	Homo sapiens	peptide-L-methionine + thioredoxin disulfide + H2O	-	?

Synonyms

Synonyms	Comment	Organism
MsrA	-	Homo sapiens
peptide methionine sulfoxide reductase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
dithiothreitol	-	Homo sapiens
thioredoxin	-	Homo sapiens

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Release 2023.1 (January 2023)