Cloned (Comment) | Organism |
---|---|
overexpression of His10-tagged MsrA in Escherichia coli strain BL21(DE3) | Mycoplasma genitalium |
Protein Variants | Comment | Organism |
---|---|---|
additional information | msrA disruption mutants, constructed by insertion mutagenesis, show highly increased sensitivity to H2O2 and reduced capability to adhere to host cell surfaces for infection, overview | Mycoplasma genitalium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycoplasma genitalium | MsrA is a virulence determinant for the plant pathogen required for full virulence | ? | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | Mycoplasma genitalium | stereospecific reduction of protein-bound methionine (S)-sulfoxide residues, the enzyme is involved in repair of oxidized proteins by reducing oxidized methionine residues, which is required for resistance to hydrogen peroxide and other reactive oxygen species, and for adherence to host cell surfaces | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycoplasma genitalium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His10-tagged MsrA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycoplasma genitalium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | MsrA is a virulence determinant for the plant pathogen required for full virulence | Mycoplasma genitalium | ? | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | stereospecific reduction of protein-bound methionine (S)-sulfoxide residues, the enzyme is involved in repair of oxidized proteins by reducing oxidized methionine residues, which is required for resistance to hydrogen peroxide and other reactive oxygen species, and for adherence to host cell surfaces | Mycoplasma genitalium | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | stereospecific reduction of protein-bound methionine (S)-sulfoxide residues | Mycoplasma genitalium | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MsrA | - |
Mycoplasma genitalium |
peptide methionine sulfoxide reductase | - |
Mycoplasma genitalium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Mycoplasma genitalium |