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Literature summary for 1.8.4.11 extracted from
- Identification and characterization of a putative active site for peptide methionine sulfoxide reductase (MsrA) and its substrate stereospecificity (2000), J. Biol. Chem., 275, 14167-14172.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| KCl | enzyme prefers high ionic strength, activation | Saccharomyces cerevisiae |  |
| Na2SO4 | enzyme prefers high ionic strength, activation | Saccharomyces cerevisiae | |
| NaCl | enzyme prefers high ionic strength, activation | Saccharomyces cerevisiae | |
| NaF | enzyme prefers high ionic strength, activation | Saccharomyces cerevisiae | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged wild-type and mutant enzymes in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C25S | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
| F26A | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
| F26H | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
| G24A | site-directed mutagenesis, 60% reduced activity compared to the wild-type enzyme | Saccharomyces cerevisiae |
| G28A | site-directed mutagenesis, 81% reduced activity compared to the wild-type enzyme | Saccharomyces cerevisiae |
| W27A | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.044 | - |
L-methionine (S)-sulfoxide | recombinant wild-type enzyme, pH 7.5, 37°C | Saccharomyces cerevisiae | |
| 0.12 | - |
L-methionine (S)-sulfoxide | recombinant wild-type enzyme, pH 7.5, 37°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | enzyme prefers high ionic strength, activation | Escherichia coli | |
| Na2SO4 | enzyme prefers high ionic strength, activation | Escherichia coli | |
| NaCl | enzyme prefers high ionic strength, activation | Escherichia coli | |
| NaF | enzyme prefers high ionic strength, activation | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-methionine (S)-sulfoxide + thioredoxin | Escherichia coli | FMsr is absolutely specific for the S-isomer of free methionine sulfoxide, no activity with protein bound methionine sulfoxide | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (S)-sulfoxide + thioredoxin | Saccharomyces cerevisiae | MsrA is specific for the S-isomer | L-methionine + thioredoxin disulfide + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Escherichia coli |
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
| additional information | - |
- |
Saccharomyces cerevisiae |
| 4.2 | - |
purified recombinant wild-type enzyme, substrate L-methionine (S)-sulfoxide | Saccharomyces cerevisiae |
| 43 | - |
purified recombinant wild-type enzyme, substrate dabsyl-L-methionine (S)-sulfoxide | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-methyl 4-tolyl sulfoxide + thioredoxin | - |
Saccharomyces cerevisiae | ? | - |
? | |
| (S)-methyl 4-tolyl sulfoxide + thioredoxin | FMsr is specific for the S-isomer | Escherichia coli | ? | - |
? | |
| dabsyl-L-methionine (S)-sulfoxide + thioredoxin | MsrA is specific for the S-isomer | Saccharomyces cerevisiae | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| dabsyl-L-methionine (S)-sulfoxide + thioredoxin | FMsr is specific for the S-isomer | Escherichia coli | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (S)-sulfoxide + thioredoxin | FMsr is absolutely specific for the S-isomer of free methionine sulfoxide, no activity with protein bound methionine sulfoxide | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (S)-sulfoxide + thioredoxin | MsrA is specific for the S-isomer | Saccharomyces cerevisiae | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| additional information | substrate specificity | Escherichia coli | ? | - |
? | |
| additional information | substrate specificity | Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FMsr | - |
Escherichia coli |
| methionine sulfoxide reductase | - |
Escherichia coli |
| methionine sulfoxide reductase | - |
Saccharomyces cerevisiae |
| MsrA | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
| 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
| 7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | - |
Escherichia coli | |
| thioredoxin | - |
Saccharomyces cerevisiae | |
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