Literature summary for 1.8.4.10 extracted from

Stevenson, C.E.; Hughes, R.K.; McManus, M.T.; Lawson, D.M.; Kopriva, S.
The X-ray crystal structure of APR-B, an atypical adenosine 5-phosphosulfate reductase from Physcomitrella patens (2013), FEBS Lett., 587, 3626-3632 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure is consistent with a preference for adenosine 5'-phosphosulfate. The [Fe4-S4] cluster enhances the enzyme's activity	Physcomitrium patens

Protein Variants

Protein Variants	Comment	Organism
D122G	mutant does not display improved activity with 3'-phosphoadenosine-5'-phosphosulfate and shows significantly reduced activity with 5'-adenylylsulfate as the substrate	Physcomitrium patens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0058	-	5'-adenylyl sulfate	mutant D122G, pH not specified in the publication, temperature not specified in the publication	Physcomitrium patens
0.0069	-	5'-adenylyl sulfate	wild-type, pH not specified in the publication, temperature not specified in the publication	Physcomitrium patens
0.04	-	3'-phosphoadenosine-5'-phosphosulfate	mutant D122G, pH not specified in the publication, temperature not specified in the publication	Physcomitrium patens
0.049	-	3'-phosphoadenosine-5'-phosphosulfate	wild-type, pH not specified in the publication, temperature not specified in the publication	Physcomitrium patens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
64000	-	gel filtration	Physcomitrium patens
71000	-	dynamic light scattering	Physcomitrium patens

Organism

Organism	UniProt	Comment	Textmining
Physcomitrium patens	Q8L5D0	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenosine-5'-phosphosulfate + thioredoxin	substrate of phosphoadenylyl-sulfate reductase, EC 1.8.4.8	Physcomitrium patens	? + thioredoxin disulfide	-	?
5'-adenylyl sulfate + thioredoxin	-	Physcomitrium patens	AMP + sulfite + thioredoxin disulfide	-	?

Subunits

Subunits	Comment	Organism
dimer	crystallization data	Physcomitrium patens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0001	-	3'-phosphoadenosine-5'-phosphosulfate	mutant D122G, pH not specified in the publication, temperature not specified in the publication	Physcomitrium patens
0.0002	-	3'-phosphoadenosine-5'-phosphosulfate	wild-type, pH not specified in the publication, temperature not specified in the publication	Physcomitrium patens
0.015	-	5'-adenylyl sulfate	mutant D122G, pH not specified in the publication, temperature not specified in the publication	Physcomitrium patens
0.333	-	5'-adenylyl sulfate	wild-type, pH not specified in the publication, temperature not specified in the publication	Physcomitrium patens

Cofactor

Cofactor	Comment	Organism	Structure
[4Fe-4S]-center	-	Physcomitrium patens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.0034	-	3'-phosphoadenosine-5'-phosphosulfate	mutant D122G, pH not specified in the publication, temperature not specified in the publication	Physcomitrium patens
0.0037	-	3'-phosphoadenosine-5'-phosphosulfate	wild-type, pH not specified in the publication, temperature not specified in the publication	Physcomitrium patens
2.6	-	5'-adenylyl sulfate	mutant D122G, pH not specified in the publication, temperature not specified in the publication	Physcomitrium patens
48.3	-	5'-adenylyl sulfate	wild-type, pH not specified in the publication, temperature not specified in the publication	Physcomitrium patens

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Release 2023.1 (January 2023)