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Literature summary for 1.8.4.10 extracted from

  • Paritala, H.; Carroll, K.S.
    A continuous spectrophotometric assay for adenosine 5-phosphosulfate reductase activity with sulfite-selective probes (2013), Anal. Biochem., 440, 32-39 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis development of a continuous spectrophotometric method for measuring APR activity by using sulfite-selective colorimetric or off-on fluorescent levulinate-based probes. The APR activity can be followed by monitoring the increase in absorbance or fluorescence of the resulting phenolate product Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0109
-
5'-adenylyl sulfate levulinate/rhodol-based assay, pH 7.5, 22°C Mycobacterium tuberculosis
0.015
-
5'-adenylyl sulfate levulinate/-hydroxy-4-methyl-2H-chromen-2-one-based assay, pH 7.5, 22°C Mycobacterium tuberculosis
0.0243
-
5'-adenylyl sulfate radioactive assay, pH 7.5, 22°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-adenylyl sulfate + thioredoxin
-
Mycobacterium tuberculosis AMP + sulfite + thioredoxin disulfide
-
?

Synonyms

Synonyms Comment Organism
APS reductase
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.058
-
5'-adenylyl sulfate levulinate/rhodol-based assay, pH 7.5, 22°C Mycobacterium tuberculosis
0.08
-
5'-adenylyl sulfate levulinate/-hydroxy-4-methyl-2H-chromen-2-one-based assay, pH 7.5, 22°C Mycobacterium tuberculosis
0.13
-
5'-adenylyl sulfate radioactive assay, pH 7.5, 22°C Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.53
-
5'-adenylyl sulfate levulinate/-hydroxy-4-methyl-2H-chromen-2-one-based assay, pH 7.5, 22°C Mycobacterium tuberculosis
0.53
-
5'-adenylyl sulfate levulinate/rhodol-based assay, pH 7.5, 22°C Mycobacterium tuberculosis
0.55
-
5'-adenylyl sulfate radioactive assay, pH 7.5, 22°C Mycobacterium tuberculosis