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Literature summary for 1.8.3.4 extracted from

  • Gould, W.D.; Kanagawa, T.
    Purification and properties of methyl mercaptan oxidase from Thiobacillus thioparus TK-m (1992), J. Gen. Microbiol., 138, 217-221.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Thiobacillus thioparus
CuSO4
-
Thiobacillus thioparus
cyanide
-
Thiobacillus thioparus
ethyl mercaptan
-
Thiobacillus thioparus
methanethiol
-
Thiobacillus thioparus
NEM
-
Thiobacillus thioparus
Sulfide no inhibition Thiobacillus thioparus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0313
-
methanethiol
-
Thiobacillus thioparus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
gel filtration Thiobacillus thioparus
40000
-
1 * 40000, SDS-PAGE Thiobacillus thioparus

Organism

Organism UniProt Comment Textmining
Thiobacillus thioparus
-
-
-
Thiobacillus thioparus TK-m
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thiobacillus thioparus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.27
-
-
Thiobacillus thioparus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethyl mercaptan + O2 + H2O
-
Thiobacillus thioparus acetaldehyde + H2S + H2O2
-
?
ethyl mercaptan + O2 + H2O
-
Thiobacillus thioparus TK-m acetaldehyde + H2S + H2O2
-
?
methanethiol + O2 + H2O
-
Thiobacillus thioparus formaldehyde + H2S + H2O2
-
?
methanethiol + O2 + H2O
-
Thiobacillus thioparus TK-m formaldehyde + H2S + H2O2
-
?
sulfide + O2 no activity Thiobacillus thioparus sulfur + H2O2
-
?
sulfide + O2 no activity Thiobacillus thioparus TK-m sulfur + H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Thiobacillus thioparus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9.6
-
Thiobacillus thioparus